牙龈卟啉单胞菌与牙周基础治疗关系的实验研究
Association between Porphyromonas gingivalis and scaling and root planning ther apy
作者：杨禾 徐屹 赵蕾 孟姝 吴亚菲
Author：YANG He1, XU Yi2, ZHAO Lei1, MENG Shu1, WU Ya - fei2
收稿日期：2008-04-25 年卷（期）页码：2008,26(02):147-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：牙周炎,牙龈卟啉单胞菌,牙周洁刮治术,实时荧光定量聚合酶链反应,
Key words：periodontitis,Porphyromonas gingivalis,scaling and root planning,real - time polymerase chain reaction,
基金项目：
国家“十五”科技攻关资助项目（2004BA720A26）
中文摘要
目的应用TaqMan实时荧光定量聚合酶链反应法检测慢性牙周炎患者牙周洁刮治术（SRP）治疗前后龈下菌斑中牙龈卟啉单胞菌（P. gingivalis）的变化，分析P. gingivalis与SRP疗效之间的关系，探讨应用实时荧光定量聚合酶链反应监测和评价SRP的可能性。方法选择20例中重度慢性牙周炎患者为研究对象，检查SRP治疗前后的临床指标，包括探诊深度（PD）、临床附着丧失（CAL）和探诊出血（BOP）；采集SRP治疗前后的龈下菌斑共142个样本，应用TaqMan实时荧光定量聚合酶链反应检测样本中的P. gingivalis。构建含有P. gingivalis扩增片段的重组质粒，建立定量标准。结果慢性牙周炎患者SRP治疗后PD、CAL和BOP均明显下降（P0.05），但治疗后治疗有效位点P. gingivalis数量减少程度明显大于治疗无效位点（P
英文摘要
Objective Porphyromonas gingivalis（P. gingivalis）is considered to be major putative periodontal pathogens. The purpose of the study was to evaluate P. gingivalis and clinical effects of scaling and root planning（SRP） in 20 subjects after 3 months. Methods Twenty periodontitis patients were selected. The mean age was（44.33 ± 13.86） years old. Clinical assessments of probing depth（PD）, clinical attachment loss（CAL） and bleeding on probing （BOP）were made prior to SRP and at 3 months post- therapy. Subgingival plaque samples were taken at each visit and analyzed using TaqMan real - time polymerase chain reaction for the presence and levels of P. gingivalis. The quantification for P. gingivalis was also performed with the help of the species- specific primers/probes and the serial dilution of the plasmid standards. Results Mean probing depth, mean clinical attachment loss and bleeding on probing showed sig- nificant reduction at 3 months（P
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