流体切应力增加骨髓来源破骨细胞内游离钙离子浓度的研究
Fluid shear str ess incr eases the Ca2 + concentr ation in bone - mar row der ived osteoclast - like cells
作者：陈明 梁星 温颖 白保晶 黄梦鹿 高卫民
Author：CHEN Ming1，2, LIANG Xing3, WEN Ying1, BAI Bao- jing1, HUANG Meng- lu1, GAO Wei- min1
收稿日期：2007-08-25 年卷（期）页码：2007,25(04):412-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：破骨细胞,激光扫描共聚焦显微镜,流体切应力,
Key words：osteosteoclast,laser scanning confocal microscope,fluid shear stress,
基金项目：
国家自然科学基金资助项目（30271428和30672345）
中文摘要
目的研究流体切应力对培养于载玻片上的破骨细胞内Ca2+浓度的影响。方法应用激光扫描共聚焦显微镜和Fluo- 3/AM荧光探针标记技术，测定加力前后破骨细胞内游离Ca2+的荧光强度，获得的图像在计算机上用图像分析软件进行比较分析。结果在37 ℃、Fluo- 3/AM终浓度为10 μmol/L的条件下，破骨细胞可获得良好的标记效果，与未加力的对照组相比，流体切应力使破骨细胞内Ca2+荧光信号的平均强度值显著增加。结论体外培养于载玻片上的破骨细胞内Ca2+浓度对流体切应力敏感，Ca2+可介导流体切应力对破骨细胞功能的调节。
英文摘要
Objective To study the change of Ca2+ density in cultured osteoclast- like cells in response to fluid shear stress. Methods Laser scanning confocal microscope and fluorescent probe were used to detect the free Ca2+ in osteoclast- like cells before and after undergoing fluid shear stress. The images were analyzed and compared with image software. Results At 37 ℃ the free Ca2+ in osteoclast- like cells could be labelled effectively with 10 μmol/L Fluo- 3/AM. Compared with contol group, the average intensity of Ca2+ fluorescent signal in osteoclast- like cells undergoing fluid shear stress increased significantly. Conclusion The Ca2+ concentration in bone- marrow derived osteoclast- like cells is sensitive to fluid shear stress, which suggests osteoclast - like cells modulate their function in response to fluid shear stress through the change of free Ca2+ concentration.
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