Objective To study the effect of platelet - rich plasma（PRP） and latissimus dorsi myofascia with blood vessel on vascularization of tissue engineered bone in dogs. Methods Bone marrow stromal cells（BMSCs） were isolated from iliac bone of dogs. PRP was obtained from the same dog. And demineralized bone matrix（DBM） were prepared from homologuous bone. ABCD 4 areas were divided on the back of dog. PRP/BMSCs/DBM was implanted around the vessels of lattisimus dorsi muscle in the A. PRP/BMSCs/DBM wrapped by superficial fascia in the B. BMSCs/DBM was implanted around vessels of lattisimus dorsi muscle in the C. BMSCs/DBM wrapped by superficial fascia in the D area of the same dog. 4, 8, 12 weeks after implantation, gross specimen and histology examination were made. Results Osteogenesis and blood vessel formation results were A>B>C>D area. Conclusion The results suggested that the PRP and latissimus dorsi myofascia with blood vessels could promote calcification and vascularization in tissue- engineered bone.
