Objective To explore the nursing methods during canal obturation in order to improve efficiency and quality of root canal therapy. Methods 112 teeth treated by complete root canal preparation were chosen to fill the root canal, and the main points of nursing during canal obturation were summarized. Results All of 112 teeth nursed strictly during canal obturation obtained satisfactory efficiency. No one failed after root canal therapy. Conclusion The main points of nursing during canal obturation are the aseptic technique, skilled coordination, instrument management, communication between nurses and patients.