肿瘤exosome诱导特异性T杀伤细胞的研究
Study of Induction of Tumor Specific Cytotoxic T Lymphocyte by Using Tumor-derived Exosome
作者：王东关;孙善珍;王振光;王霞
Author：WANG Dong-guan，SUN Shan-zhen，WANG Zhen-guang，WANG Xia
收稿日期：2006-04-25 年卷（期）页码：2006,24(02):160-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：Tca8113细胞,细胞毒性T淋巴细胞,树突状细胞,
Key words：Tca8113cell,cytotoxicTlymphocyte,dendriticcell,
基金项目：
高等学校博士学科点专项科研基金资助项目（20030422041）
中文摘要
目的检测癌细胞来源的exosome在体外刺激细胞毒性T淋巴细胞(CTL)特异性杀伤癌细胞的能力.方法分离纯化舌鳞癌Tca8113细胞,培养上清液中的exosome,制备癌细胞的冻融抗原(FTA),并把两者负载到从血液分离和培养的树突状细胞(DC)上,测定其诱导的CTL对Tca8113的杀伤活性,以SPCA-1人肺腺癌细胞系和95-D人巨细胞癌为对照组.结果体外FTA和exosome冲击致敏的DC能显著刺激T淋巴细胞增殖,其诱导的CTL对细胞系Tca8113具有显著的杀伤作用,在效靶比为20:1时12 h平均分别为45.19%+4.57%和43.60%+5.66%(P＜0.01);exosome冲击致敏的DC诱导的CTL对SPCA-1人肺腺癌也有明显的杀伤作用(P＜0.05),但FTA冲击致敏的DC诱导的CTL却无此功能.结论肿瘤细胞培养上清液中分离出的exosome,负载到DC上后,可以活化T细胞,使之成为抗原特异性的CTL,具有特异性的杀伤肿瘤细胞的功能,为口腔癌的免疫治疗开拓了新的途径.exosome特异的CTL也可杀死其他肿瘤细胞,说明肿瘤来源的exosome是一种可引起肿瘤消退的共同抗原.
英文摘要
Objective To investigate whether the exosomes derived from Tca8113 could induce production of tumor-specific T cells when pulsed onto dendritic cells. Methods Tca8113 cell was cultured with RPIMI640. isolated and purified the tumor-derived exosomes from the culture supernatants by ultrafiltration with Millipore centrifual filter devices; frozen and thawed Tca8113 cells to get frozen tumor antigens(FTA). The exosomes and FT A was pulsed onto DC generated from normal human peripheral blood mononuclear cell (PBMC) in vitro. The DC pulsed with FAP or exosomes cocultured with the peripheral blood lymphocytes to transform T cell into specific CTL To observe the killing and wounding activity of CTL, the CTL and Tca8113 cells were mixed at a ratio of 20 to 1, SPCA-l cells and 95-D cells was evaluated as control group. Results The CTL induced by DC pulsed with FAP or exosomes had significant activity killing Tca8113 CP
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