转录因子c-Jun和c-Fos在牙本质涎磷蛋白基因表达中的作用
Transcriptional Regulation of Dentin Sialophosphoprotein by c-Jun/c-Fos
作者：何文喜;牛忠英;赵守亮;李萍;高杰
Author：HE Wen-xi1, NIU Zhong-ying2, ZHAO Shou-liang1, Li Ping1, Gao Jie1
收稿日期：2006-02-25 年卷（期）页码：2006,24(01):67-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：牙本质涎磷蛋白,c-Jun,c-Fos,基因转录,
Key words：dentinsialophosphoprotein,c-Jun,c-Fos,genetranscription,
基金项目：
国家自然科学基金资助项目（30200315）
中文摘要
目的 研究成牙本质细胞内转录因子c-Jun和c-Fos在牙本质涎磷蛋白（DSPP）基因转录调控中的作用，
探索成牙本质细胞内DSPP基因表达的调控机制。方法 细胞免疫组化观察MDPC-23细胞内c-Jun和c-Fos蛋白分子的表达。瞬时转染和报告基因检测c-Jun和c-Fos在DSPP基因转录中的作用。结果 MDPC-23细胞表达c-Jun和c-Fos蛋白，c-Jun和c-Fos主要分布在MDPC-23细胞胞核。c-Jun或c-Fos过表达均显著抑制DSPP基因启动子活性。结论 证实成牙本质细胞内转录因子c-Jun和c-Fos参与下调DSPP基因表达。
英文摘要
bjective To investigate the role of c-Jun and c-Fos as transcriptional factors in regulation of dentin sialophosphoprotein（DSPP） gene by a promoter-luciferase reporter gene construct in odontoblast cell line MDPC-23. Methods Endogenous c-Jun or c-Fos protein was determined by immunocytochemistry. The role of c-Jun or c-Fos in transcription of DSPP was investigated in co-transfection experiments using promoter-luciferase reporter gene construct containing the sequence between -791 bp and +54 bp of mouse DSPP gene. Results c-Jun and c-Fos was expressed by MDPC-23 cells, and located in the nucleus of MDPC-23 cells. Overexpression of c-Jun or c-Fos significantly inhibited luciferase activity of DSPP promoter. Conclusion These findings suggest c-Jun and c-Fos downregulated the transcription of DSPP gene as a transcriptional factor in odontoblast.
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