整合素α_Vβ_3在下颌牵张成骨过程中表达的实验研究
Study of the Expression of IntegrinαVβ3in Mandibular Distraction Osteogenesis
作者：魏奉才;张东;刘少华;孙善珍
Author：WEI Feng-cai1,ZHANG Dong1,LIU Shao-hua1,SUNShan-zhen2
收稿日期：2005-12-25 年卷（期）页码：2005,23(06):474-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：牵张成骨,整合素,下颌骨,
Key words：distraction osteogenesis,integrin,mandible,
基金项目：
国家自然科学基金资助项目(30271349);山东省自然科学基金资助项目(Y20001C024)
中文摘要
目的 研究整合素αVβ3(ItgαVβ3)在下颌牵张成骨(DO)过程中的表达分布情况,探讨ItgαVβ3在DO中的作
用。方法 选用成年新西兰大白兔44只,随机分为3组。分别为牵张组、骨折对照组和正常对照组,各20、20、4只
动物。分别建立动物模型并于不同时期处死动物,取标本行免疫组织化学染色,观察ItgαVβ3的表达及分布情况。
采用半定量法对组织切片的着色强度分级评分,结果行统计学处理。结果 牵张及骨折组ItgαVβ3表达增强,阳性
着色主要位于血管内皮细胞(EC),定位于胞膜和胞浆,正常对照组骨组织无阳性着色。牵张组较骨折组ItgαVβ3的
表达强度大,持续时间长。结论 ItgαVβ3能够促进牵张区血管形成,改善局部供养,从而提高新骨形成的速度和质
量。
英文摘要
Objective To investigate the expression and distribution pattern of IntegrinαVβ3(ItgαVβ3) in course of distraction osteogenesis and to quest for the function of ItgαVβ3in distraction osteogenesis (DO).Methods 44 adults New Zealand white rabbits were selected and divided randomly into 3 groups: DO, bone fracture and normal group. There were 20, 20 and 4 rabbits in each group separately. Animal models were established and animals were killed at different time points. Sections were stained by IHC method. Distribution and expression pattern of ItgαVβ3were observed by semi-quantitative technique, and the results were managed with statistic methods.Results The expression of ItgαVβ3was found both in the DO and bone fracture groups. The positive stainingwas seenmainly in vascular endothelial cells on cytomembrane and in cytoplasm. The staining intensity of group of DOwas higher than that of the bone fracture group.Conclusion ItgαVβ3plays an important role in promoting the process of DO.
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