新型纳米羟基磷灰石根充糊剂的细胞毒性研究
Evaluation on Cytotoxicity of a New Nano-hydroxyapatite as Root Canal Filling Sealer
作者：李平,肖丽英,李伟,谭红,廖运茂,袁明龙
Author：LIPing1,XIAOLi-ying2,LI Wei2,TANHong1,LIAO Yun-mao2,YUANMing-long3
收稿日期：2005-10-25 年卷（期）页码：2005,23(05):427-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：纳米羟基磷灰石,根管充填糊剂,细胞毒性,
Key words：nano-hydroxyapatite,root canal sealer,cytotoxicity,
基金项目：教育部留学回国人员科研启动资金资助项目(0040305
402005)
中文摘要
目的 体外研究新型纳米羟基磷灰石(n-HA)根充糊剂的细胞毒性。方法 体外培养成骨细胞,采用碱性
磷酸酶(ALP)和Ⅰ型胶原免疫组化染色鉴定成骨细胞;采用MTT比色法,以F12培养基为对照,选用3种不同浓度
的新型n-HA根充糊剂浸提液,分别检测浸提液与成骨细胞接触1、3、5、7 d的细胞相对增殖率(RGR),以评价其细
胞毒性。结果 与对照组相比,新型n-HA根充糊剂表现出较高的细胞相对增殖率,随着浸提液浓度的降低和培养
时间的延长,细胞毒性趋于0级;相同时间点不同浓度的新型n-HA根充糊剂浸提液的吸光度值无显著性差异(P>
0·05)。结论 新型纳米羟基磷灰石根充材料无细胞毒性。
英文摘要
Objective To evaluated the cytotoxicity of a newnano-hydroxyapatite (n-HA)root canal sealer.Methods In this study, the cytotoxicity was evaluated by the method of MTT assayin vitro, and culture medium F12 as control, three concentra- tions of the soaking material cultured with mouse osteoblast separately, to test the cell relative growth rate(RGR) of every group. Results The toxicity graduation of the n-HA root canal sealer tends to 0 with the culture time increasing. The cell survival rate of n-HA root canal sealer showed high relatively. The OD value of cell was similar for the negative control and the extracts(P> 0·05).Conclusion The result indicated that n-HA root canal sealer was compatible with the testcells.
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