全托儿童口腔变形链球菌水平传播的初步研究
Study on the Horizontal Transmission of OralStreptococcus mutansin Children
作者：邹静,尚冉,凌均棨,周学东
Author：ZOUJing1,SHANG Ran2,LING Jun-qi1,ZHOUXue-dong3
收稿日期：2005-10-25 年卷（期）页码：2005,23(05):388-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：变形链球菌,基因型,随机引物聚合酶链反应法,DNA指纹分析,
Key words：Streptococcus mutans,genotype,arbitrarily primed polymerase chain reaction,DNA fingerprinting,
基金项目：中国博士后科学基金资助项目(2005037617)
中文摘要
目的 通过对幼儿园全托儿童之间口腔变形链球菌水平传播的研究,为儿童龋病预防提供新的思路。方
法 选择24名3~4岁全托儿童为研究对象,其中无龋组和有龋组各12名。采用无菌牙签采集牙面菌斑,厌氧培
养48 h,根据变形链球菌的典型形态挑取单个菌落进行次代纯培养,经形态学和微量生化鉴定后将分离的菌株行
AP-PCR扩增,对来自不同个体基因型相似的菌株再进行DNA指纹分析。结果 24名受检儿童中,变形链球菌检出
率为66·7%,无龋组和有龋组检出率分别为58·3%和75·0%。经AP-PCR扩增,24名儿童口腔中检测出29个不同
基因型的变形链球菌,其中携带2个以上基因型儿童占45·8%。经DNA指纹分析,有2种基因型在11名全托儿童
口腔中有重复检出。结论 变形链球菌在全托儿童口腔中可能存在水平传播。
英文摘要
Objective To find out horizontal transmission of oralStreptococcus mutans(S.mutans) in nursery children through analyzing the similarity ofS.mutansgenotypes.Methods The study group included 24 nursery children between 3 and 4 years of age. Dental plaque sampleswere collectedwith sterile toothpick and cultured onMSB plates for48 h. IndividualStreptococcus mu- tansgroup colonies representative of the colonial morphologies were subcultured on TPY plates. These strains were identified to species level biochemically. AP-PCR fingerprinting was preformed after identification.S.mutansisolates from different children with very similar fingerprinting profiles were examined by chromosomal DNA fingerprinting analysis.Results Streptococcus mu- tansgroup were isolated in oral cavities of 66.7% children, 58.3% in caries-free and 75.0% in caries children. A total of 46 S.mutansisolates from 24 subjects were analyzed by AP-PCR, and 29 different amplitypes were identifyied, 45.8% carried two genotypes. There were 2 genotypes ofS.mutansisolated repeatedly among 12 nursery children.Conclusion The presence of matching genotypes ofS.mutansamong nursery children suggests horizontal transmission.
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