Runx2/Cbfa1在出生后小鼠牙髓牙本质复合体形成中的作用研究
Expression of Runx2/Cbfa1 in the Developing Pulpo-dentinal Complex of Postnatal Mice
作者：潘克清,李纾,杨丕山,赵艳红
Author：PANKe-qing,LI Shu,YANG Pi-shan,ZHAO Yan-hong
收稿日期：2005-10-25 年卷（期）页码：2005,23(05):373-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：Runx2/Cbfa1,免疫组织化学,牙髓牙本质复合体,发育,
Key words：Runx2/Cbfa1,immunohistochemistry,pulpo-dentinal complex,development,
基金项目：
国家自然科学基金资助项目(30371537);山东省中青年科学家奖励基金资助项目(02BS096)
中文摘要
目的 探讨Runx2/Cbfa1在出生后小鼠成牙本质细胞分化及牙本质形成中时间和空间的分布特点及意
义。方法 采用免疫组化SABC法检测Runx2/Cbfa1在出生后BALB/c小鼠牙本质、成牙本质细胞及牙髓细胞中不
同发育时期的表达及特点。结果 Runx2/Cbfa1在成牙本质细胞的发育成熟过程中具有时空特异性,在牙根尚未发
育之前的牙胚钟状晚期,Runx2/Cbfa1只在前期牙本质中有表达,在牙髓细胞及成牙本质细胞中皆为阴性;约从第11
天开始,随着牙根的发育,Runx2/Cbfa1在根部成牙本质细胞、牙髓细胞中表达为阳性,在冠部成牙本质细胞中表达
为阴性。结论 Runx2/Cbfa1参与牙本质的形成和矿化及成牙本质细胞和牙髓细胞的发育,可能对它们起重要作
用。
英文摘要
Objective To study the expression of Runx2/Cbfa1 in the developing dentin and differentiating odontoblasts.Meth- ods A postnatal mice teeth developing model was built histologically. Immunohistochemical technique was adopted to determine the expression of Runx2/Cbfa1 in the developing pulpo-dentinal complex in mice.Results Runx2/Cbfa1 was merely present in predentin in the exact and before the 11th day′s postnatal stages. Meanwhile, it was positively located in odontoblasts and dental pulp cells in root region, but negatively in coral part after the 11th day′s stages.Conclusion Runx2/Cbfa1 may play an impor- tant role in the deposing of tooth dentin and in the differentiating of odontoblasts and pulp cells.
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