肝细胞生长因子对牙髓细胞增殖的影响
Effect of Hepatocyte Growth Factor on the Proliferation of Dental Pulp Cell
作者：叶玲,凌均棨,彭栗,谭红,周学东
Author：YE Ling1,LING Jun-qi1,PENG Li2,TAN Hong2,ZHOUXue-dong2
收稿日期：2005-06-25 年卷（期）页码：2005,23(03):198-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：肝细胞生长因子,牙髓细胞,细胞增殖,细胞周期,
Key words：hepatocyte growth factor,dental pulp cell,cell proliferation,cell cycle,
基金项目：
中文摘要
目的 探讨肝细胞生长因子对牙髓细胞生长增殖的影响。方法 以体外培养的牙髓细胞为实验对象,以
不同浓度肝细胞生长因子作用于牙髓细胞,通过二甲基噻唑二苯基四唑溴盐比色法对细胞的增殖情况进行研究,
用流式细胞术对细胞的周期进行分析。结果 1~200μg/L的肝细胞生长因子可明显促进牙髓细胞的增殖
(P
英文摘要
Objective to studythe effect of hepatocyte growth factor on the proliferation of dental pulp cell.Methods the 4th generation dental pulp cell cultured in vitro was used as target cell. 1~200μg/L hepatocyte growth factor was added in the test group while the pure cell culture DMEMas control. TheMTTmethod and flowcytometrywere applied to assay the proliferation and cell cycle of dental pulp cell of different groups.Results 1~200μg/L hepatocyte growth factor showed promoting effect to the proliferation of pulp cell since the 5th day (P
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