体外诱导的成骨细胞与金属材料界面的初步研究
Inducement and Proliferation of MSCsin vitroand Preliminary Study on Interface between MSCs and Metal Materials
作者：郁春华,张保卫,张修银,高建新
Author：YUChun-hua1,ZHANGBao-wei1,ZHANGXiu-yin1,GAOJian-xin2
收稿日期：2005-06-25 年卷（期）页码：2005,23(03):194-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：骨髓基质干细胞,细胞培养,成骨作用,界面,应变,
Key words：marrowstemcells,cellculture,osteogenisis,interface,strain,
基金项目：国家自然科学基金资助项目(19602013)
中文摘要
目的 研究体外诱导成骨细胞的特性及其与金属材料贴附形成的界面。方法 采用成年犬骨髓基质干
细胞进行体外成骨诱导培养并置入金属材料,借助相差显微镜、生化检测、透射电镜、扫描电镜等手段进行分析。
结果 体外成骨诱导的骨髓基质干细胞显示出强大的成骨活性,并无论在光滑表面还是粗糙表面都能与材料贴附
形成较好界面,且在应变量最大的张应力区分布较紧密。结论 经诱导的骨髓基质干细胞可作为成骨细胞的来
源,并能在金属材料表面贴附形成界面,且实验所采用的喷砂粗化处理对其影响并不显著,但材料的应变程度及应
力性质可能与之有一定的关系。
英文摘要
Objective The aims of the present studywere to assess the characteristics ofMSCswhichwere induced and prolif- eratedin vitro, then to learn the interfaces between them and metal materials.Methods Adult dogswere selected as experimen- tal animals, the MSCs (marrow stem cells) were aspirated from the femur, which were induced to the phenotype of OB (osteo- blast), then to proliferatedin vitrowith metal materials. Using biochemical tests, phase contrast microscopy, transmit electromi- croscope, scanning electromicroscope to detect MSCs and the image of interface.Results MSCs had powerful osteogenic pheno- type when they were inducedin vitro, and they also attached to the metal materials. And there were more cellswhich attached on tensile stress area than other areas.Conclusion The MSCs inducedin vitrocould be used as source of osteoblast. The affects of air-abrasion with alumina are not significant, but they have relation to the extent of strain and property of stress. [Key words] marrow stem cells|cell culture|osteogenisis|interface|strain
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