纳米羟磷灰石/聚酰胺66对成骨细胞生物学作用的实验研究
Biological Effects of Nano-hydroxyapatite/Polyamide 66 on the Osteoblast
作者：叶玲,苏勤,周学东
Author：YELing, SUQin, ZHOUXue-dong
收稿日期：2005-04-25 年卷（期）页码：2005,23(02):142-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：纳米羟磷灰石/聚酰胺66,实时定量PCR,成骨细胞,
Key words：nano-hydroxyapatite/polyamide 66,quantitative reverse transcriptase-PCR,osteoblast,
基金项目：四川省科委重点资助项目(02SG022-001)
中文摘要
目的 研究新型纳米根管充填材料纳米羟磷灰石/聚酰胺66(nHA-PA66)对成骨细胞的生物学作用。方法
以含nHA-PA66的细胞培养基(DMEM)浸提液作用于实验组细胞,DMEM培养基作用于对照组细胞,采用MTT比
色法检测nHA-PA66对成骨细胞生长的影响;酶联法检测细胞ALP活性的变化;QRT-PCR测量细胞骨钙素基因表达
的变化。结果 实验组细胞的相对增殖率在98%~106%之间,且无量效关系,实验组与对照组间无显著性差异
(P>0·05);实验组和对照组细胞的ALP活性及骨钙素基因表达相似,皆无显著性差异(P>0·05)。结论 nHA-
PA66对成骨细胞的生长和功能无不良影响,具有骨细胞相容性。
英文摘要
Objective To evaluate the biological effects of nano-hydroxyapatite/polyamide 66(nHA-PA66) on the growth and activity of osteoblast.Methods MTTassaywas used to determine the growth of osteoblast, enzymatic measure was used to deter- mine the activity of ALP and quantitative RT-PCR (QRT-PCR) to evaluate the changes of osteoclacin mRNA expression in os- teoblasts treated by DMEM eluate of nHA-PA66.Results Osteoblasts of different test groups demonstrated relative proliferation rate ranging from 98%~106% without dose-dependent effect. The ALP activity and osteocalcin mRNA expressionwere similar in test and control groups (P>0.05).Conclusion nHA-PA66 has no negative effects on the osteoblast and its osteoblast-compati- bility is proved.
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