大鼠骨髓间充质干细胞复合藻酸盐体内异位成软骨的初步研究
Study on the Chondrogenesis of the Compound of Alginate Gelatin and Bone Marrow Stromal Cellsin vivo
作者：刘磊,陈润良,田卫东,闫征斌,陈希哲,李声伟,王涛
Author：LIULei, CHENRun-liang,TIANWei-dong,YANZheng-bin,CHENXi-zhe,LISheng-wei,WangTao
收稿日期：2005-02-25 年卷（期）页码：2005,23(01):60-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：骨髓间充质干细胞,藻酸盐,软骨,组织工程,
Key words：mesenchymal stromal cells(MSCs),alginate gelatin,cartilage,tissue engineering,
基金项目：国家自然科学基金资助项目(30100210)
中文摘要
目的 探讨以藻酸盐为支架材料,经体外软骨向诱导的大鼠骨髓间充质干细胞为种子细胞,在体内异位形成组织工程化软骨的可能性。方法 32只雄性SD大鼠随机平均分为实验组和对照组两组。实验组分离培养自体骨髓间充质干细胞,传至第3代经转化生长因子β等诱导因子诱导10 d后与藻酸钠复合,并滴加氯化钙使其成凝胶状后注入大鼠背部皮下。对照组只注入藻酸盐。术后4周、8周取材进行苏木精-伊红、奥新蓝染色及透射电镜观察。结果 实验组术后8周肉眼可见软骨形成,苏木精-伊红、奥新蓝染色发现有大量软骨形成,软骨内可见细胞团,软骨细胞周围基质丰富,藻酸盐降解明显,未降解的藻酸盐松散的分布于软骨间。对照组未见软骨样组织形成。结论 经软骨向诱导的自体骨髓间充质干细胞结合藻酸盐凝胶可在体内异位形成较理想的组织工程化软骨。
英文摘要
Objective To investigate the chondrogenisis by alginate gelatin and rats′bone marrowstromal cells(BMSCs) chon- drogenicly inducedin vitro.Methods Thirty-two male adult SD ratswere assigned randomly to experimental and control groups. In experimental group, bone marrowwas obtained from the right tibias of all the rats. After expanding and culturing 3 passages, induced BMSCs by chondrogenic culture medium for 10 days. Suspended induced cells in alginate gelatin, and injected the com- plex into the hypodermic tissue of the backs of rats autogenously. In control group only alginate gelatins were injected. The grafts were taken out for examinations 4 and 8 weeks after the operations.Results Considerable cartilage appeared in experimental group 8 weeks after operations. RegularHE staining and alcian blue staining showed a great deal of cartilage holding chondrocyte masses surrounded by abundant matrix. Alginate gelatin decompounded obviously, and the rest distributed among newly formed cartilage. No cartilage appeared in control group all through.Conclusion BMSCs and alginate gelatin have a beautiful future in cartilage tissue engineering.
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