小沟聚合物探针实时检测变形链球菌和远缘链球菌
MGB Probes DetectStreptococcus MutansandStreptococcus Sobrinusin Real Time
作者：凌均棨,林家成,唐志英,陈罕,高燕
Author：LING Jun-qi,LINJia-cheng,TANG Zhi-ying,CHENHan,GAO Yan
收稿日期：2005-02-25 年卷（期）页码：2005,23(01):26-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：变形链球菌,远缘链球菌,小沟聚合物探针,儿童猛性龋,实时检测,
Key words：Streptococcus mutans;Streptococcus sobrinus,minor groove binder probes,rampant caries,real time detec- tion,
基金项目：
中文摘要
目的 寻求一种快速检测变形链球菌(S.mutans)和远缘链球菌(S.sobrinus)的方法,研究变形链球菌群在儿童猛性龋患者口中的分布。方法 设计并合成针对S.mutans和S.sobrinus葡糖基转移酶基因(gtf)的特异性引物和小沟聚合物探针(MGB探针),对9株变形链球菌群参考菌株直接提取DNA及扩增纯培养后提取DNA分别进行检测,比较二者检测结果的异同。采集92例猛性龋儿童菌斑样本,用MGB探针进行实时检测。结果 采用MGB 探针可以特异性地鉴别S.mutans和S.sobrinus,直接检测和扩增纯培养后的定性检测结果完全一致,前者荧光出现的时间略迟。92例猛性龋患儿中S.mutans检出率为96.7%,S.sobrinus检出率为32.6%,所有检出S.sobrinus的菌斑样本均可检出S.mutans。结论 采用特异性MGB探针方法可以对菌斑中S.mutans和S.sobrinus进行实时检测, 提高检测效率。
英文摘要
Objective To detect and distinguishStreptococcus mutans(S.mutans) andStreptococcus sobrinus(S.sobrinus) quickly in epidemiology and investigate the distribution ofS.mutansin the oral of children with rampant caries.Methods De- signed minor groove binder (MGB) probes according to the gtf gene ofS.mutansandS.sobrinus. Detected 9 reference strains of Streptococcus mutansgroup byMGB probes in real time and after cultivation. Evaluated the results of these twomethods. 92 dental plaques from pre-school children with rampant caries were detected in real time with MGB probes.Results The primers could amplify the target sequences specificity and distinguishedS.mutansandS.sobrinusfrom each other using MGB probes. Though the fluorescence occurred earlier inS.mutansthan inS.sobrinus, they had the same results in nature. In 92 children with ram- pant caries, the detective ratio ofS.mutanswas 96.7% and that ofS.sobrinuswas 32.6%. All the sampleswhich could detect S.sobrinuswere positive forS.mutans.Conclusion The primers and probe designed fromgtf genes ofS.mutansandS.sobri- nuscan amplify the target sequence and distinguish them from each other in real time.
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