膜联蛋白I和胞浆磷脂酶A_2在小鼠腭突融合中的表达
Observing the Distribution of Annexin I and cPLA2in the Palatal Process of Mice by Immunohistochemical Staining
作者：王,石冰,左晖,黄磊
Author：WANG Yan1,SHI Bing2,ZUOHui1,HUANGLei1
收稿日期：2005-02-25 年卷（期）页码：2005,23(01):8-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：膜联蛋白,胞浆磷脂酶A2,腭裂,免疫组化染色,
Key words：Annexin I,cytosolic phospholipose A2,cleft palate,immunohistochemical staining,
基金项目：国家自然科学基金资助项目(39770801)
中文摘要
目的 观察膜联蛋白I(Annexin I)和胞浆磷脂酶A2(cPLA2)在地塞米松致畸敏感性小鼠腭胚中的分布情
况。方法 沿小鼠胚胎头部的冠状平面切取切片,获得10周龄近交系小鼠妊娠的第14~16天的腭胚突。用免疫
组化染色检测Annexin I和cPLA2的分布情况。结果 在小鼠胚胎腭突融合前后,Annexin I、cPLA2的免疫组织化学
染色在腭突上皮和间充质细胞中都为阳性,并且染色强弱呈时相性变化。结论 Annexin I和cPLA2在胚胎腭部发
育进程中起一定的调节作用,可能是地塞米松诱导发生腭裂的重要介质。
英文摘要
Objective To observe the distribution of Annexin I and cytosolic phospholipose A2(cPLA2) in the palatal process of dexamethasome teratogenerated mice.Methods Cutting along the coronary plane of the heads of fetal mice to obtain palate in the 14th, 15th, 16th day of gestation by 10-week-old in-bred mice. The distribution of Annexin I and cPLA2was checked by im- munohistochemical staining.Results Duringthe fusing of the palatal processes, the staining ofAnnexin I and cPLA2 was positive in the epithelial and mesenchymal cells of the palatal process, and the intensity of staining changed with time.Conclusion An- nexin I and cPLA2can modulate the development of fetal palate to some extent, and theymay be importantmediators in the devel- opment of cleft palate induced by dexamethasone.
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