周期性牵张体外培养面颌成肌细胞肌浆网Ca~(2+)-Mg~(2+)ATP酶活性及其mRNA变化的研究
Changes of Sarco(endo)plasmic Reticulum Ca2+-Mg2+-ATPase Activity of Cultured Myoblast Induced by Cyclic Stretch
作者：车晓霞1,曾 宏2,罗颂椒3
Author：CHEXiao-xia1,ZENGHong2,LUO Song-jiao3
收稿日期：2004-08-25 年卷（期）页码：2004,22(04):281-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：牵张,成肌细胞,Ca2+-Mg2+ATP酶,
Key words：stretch,myoblast,Ca2+-Mg2+ATPases,
基金项目：国家自然科学基金资助项目(30171024)
中文摘要
目的 观察牵张引起的成肌细胞内Ca2+-Mg2+ATP酶活性及mRNA变化,探求口腔正畸功能矫形治疗中肌肉改建机理,为正畸临床治疗及防止复发提供理论性指导。方法 采用四点弯曲加力装置,使体外培养的SD大鼠面颌部成肌细胞发生牵张变形后,无机磷-钼酸铵直接比色法测定加力后不同时段Ca2+-Mg2+ATP酶活性,RT- PCR测定肌浆网Ca2+-Mg2+ATP酶mRNA变化。结果 SD大鼠体外培养成肌细胞在加力变形后的4 h内Ca2+- Mg2+ATP酶活性显著降低,8 h到24 h期间明显升高,48 h基本接近对照组水平;此外,RT-PCR结果显示成肌细胞受牵张力后Ca2+-Mg2+ATP酶mRNA水平在各时间段组均较对照组升高,其中以2 h和48 h段组升高最明显。结论 随着受力时间的延长,成肌细胞发生了适应性变化,Ca2+-Mg2+ATP酶活性变化的控制发生在基因翻译前水平,Ca2+-Mg2+ATP酶活性及其mRNA变化会直接影响对细胞内游离Ca2+浓度的调节,继而引起细胞内发生的一系列生物学反应的变化。
英文摘要
Objective To evaluate the effect of stretch on sarco(endo)plasmic reticulum Ca2+-Mg2+ATPases activity and mR- NA level and study the remodeling reaction of muscle in a variety of mechanical environments.Methods Myoblast from maxillo- facial skeletal muscle of one-week-old male Sprague-Dawley ratwas cultured and stretched cyclicly using a four-point bend device. Inorganic Phosphorus test was used to compare the activity of Ca2+-Mg2+ATPases of myoblast before and after stretch. RT-PCR was also used to observe the Ca2+-Mg2+ATPases mRNA level.Results The activity of Ca2+-Mg2+ATPases of myoblast down regulated significantly in 4 hours. During the period of 8 hours to 24 hours, up-regulation followed then returned to control level at the 48 hour point. RT-PCR showed that Ca2+-Mg2+ATPases mRNA level were elevated by stretch, particularly at 2 hour and 48 hour point.Conclusion The results suggested a transcriptional control of Ca2+-Mg2+ATPases activitywas involved in the muscle remodeling process induced by stretch.
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