腹膜腔注射肝素对ACC-M细胞在裸鼠肺、肝组织分布的影响
The Effect of Intraperitoneal Heparin on Distribution of ACC-M in Lung and Liver
作者：王新军1,陈万涛2,周正炎1,马衍青3,周晓健2
Author：WANG Xin-jun1, CHEN Wan-tao2, ZHOUZheng-yan1, MAYan-qing3,ZHOUXiao-jian2
收稿日期：2004-06-25 年卷（期）页码：2004,22(03):246-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：肝素,ACC-M细胞,粘附,
Key words：heparin,ACC-M,adhesion,
基金项目：国家自然科学基金资助项目(39970794)
中文摘要
目的 通过观察腹膜腔注射肝素对ACC-M细胞在裸鼠肺、肝组织的分布特点,研究其对腺样囊性癌肺高转移细胞肺粘附的抑制作用。方法 实验裸鼠腹腔注射肝素后,经尾静脉注入氚标胸腺嘧啶核苷(3HTdR)标记 ACC-M细胞,分别检测注射后2 h、6 h、18 h肺、肝组织单位重量的每分钟同位素脉冲数(CPM)。结果 裸鼠单位重量肺组织3H-ACC-M在不同时间由高到低依次是空白对照组2 h、6 h、18 h,肝素组6 h、2 h、18 h。相同时段肝素组明显低于对照组,两组间有显著性差异(P0·05)。结论 肝素腹腔注射后可减少肺组织内ACC-M细胞数量,但对相同时段肝组织内ACC-M细胞数量无显著影响,肝素可能在抑制腺样囊性癌肺转移中发挥作用。
英文摘要
Objective To investigate the effect of intraperitoneal heparin on the adhesion of highly lungmetastasic adenoid cys- tic carcinoma cell line (ACC-M) in lung.Methods 3HTdR labeled ACC-M cells were injected by intravenous infusion after in- traperitoneal injection with 200 units heparin. 4 mice of each group were killed at 2 h, 6 h and 18 h after infusion. The relative radioactivity (CPM) in lung and liverwas detected.Results 3H-activity pergram in lung of heparin groupwas lowerthan control at the same time. There was significant difference between the two groups (P0·05).Conclusion The results of this study suggest that the highly lungmetastasis characteristic of ACC-M may be partially inhabited by the use of intraperitoneal heparin.
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