小鼠牙胚的鸡胚培养模型的建立
Establishment of a Culture System of Chick Embryo for Mouse Tooth Germ Development
作者：闫征斌,田卫东,刘磊,侯景秋,陈希哲,李志勇
Author：YAN Zheng-bin,TIAN Wei- dong, LIULei, HOUJin-qiu,CHENXi-zhe,LI Zhi-yong
收稿日期：2004-06-25 年卷（期）页码：2004,22(03):232-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：小鼠,牙胚,鸡胚,体内培养,
Key words：mouse,tooth germ,chick embryo,culturein vivo,
基金项目：
国家重大基础研究前期研究专项(2002CCC00700);教育部优秀青年教师教学科研奖励计划资助项目(B52003682)
中文摘要
目的 采用鸡胚建立一种新的小鼠牙胚体内培养模型。方法 取15 d龄的昆明胎鼠下颌第一磨牙牙胚, 将其用钨丝固定于孵化4~5 d(翼芽期)的鸡胚内,继续孵化10 d后,终止培养并取材,进行苏木精-伊红染色,常规组织学观察。结果 小鼠牙胚在鸡胚内可继续生长发育,从帽状期进入到钟状末期,镜下可见分化成熟的成牙本质细胞、成釉细胞和分泌的基质。结论 以翼芽期鸡胚作为小鼠牙胚体内培养的宿主,可以保持其发育,再现牙齿形成过程中的细胞分化、形态发生的生理过程。
英文摘要
Objective To establish a new culture systerm for mouse tooth germs in chick embryo.Methods The mandibular first molar germ fragments of 15 embryonic days′Kunming mouse embryo were implanted into the lateral mesenchyme of 4~5 days′chick embryo wing buds in ove. Eggs were reincubated and implanted tissues were examined by histochemistry.Results The cultured tooth germ development continued from cap stage to latest bell stage. The ameloblast and the odontoblast all differen- tiated maturely and secreted matrix.Conclusion 4~5 days′wing buds chick embryo could serve as developing the mouse tooth germs and demonstrate well physiological process of differentiation and morphogenesis.
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