重组人骨形成蛋白-2促进兔下颌牵张成骨的研究
Recombinant Human BMP-2 Accelerates Bone Formation of Mandibular Distraction Osteogenesis in Rabbits
作者：王志国,胡静,邹淑娟,李继华,高占巍,王大章
Author：WANG Zhi-guo, HUJing, ZOUShu-juan, LIJi-Hua, GAO Zhan-wei, WANGDa-zhang
收稿日期：2004-06-25 年卷（期）页码：2004,22(03):186-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：下颌骨牵张,重组人骨形成蛋白-2,新骨生成,
Key words：mandibular osteo-distraction,recombinant human BMP-2,new bone formation,
基金项目：教育部跨世纪优秀人才基金资助(教技函2002-48)
中文摘要
目的 研究局部应用基因重组人骨形成蛋白-2(rhBMP-2)对兔下颌牵张成骨的影响。方法 在12只成年大耳白兔的双侧下颌骨前部行骨切开术,将rhBMP-2与胶原复合植入一侧下颌骨切开处,另一侧单纯植入胶原作对照。用自行研制牵张器延长双侧下颌骨6 mm,在牵张结束后第4周处死动物,取双侧牵张区新生骨痂行组织学、扫描电镜及Ca/P元素测定。结果 下颌延长后两侧牵张间隙均有新骨形成,应用rhBMP-2的一侧牵张骨痂中的新骨组织比对照侧多而成熟,钙化程度较高。结论 基因重组人骨形成蛋白-2可能有促进兔下颌牵张成骨的作用。
英文摘要
Objective To study the effects of rhBMP-2 on bone formation of mandibular distraction osteogenesis in rabbits. Methods Bilateral mandibular osteotomieswere performed in 12 mature rabbits. 5 mg rhBMP-2 withthe collagen carrierwas im- planted in the osteotomy site of one side of the mandibles. Only the collagen sponge was placed in the contra-lateral side as con- trol. The mandibles of 8 rabbitswere lengthened by 6mm using a custom-made distractor. At 4 weeks after the end of distraction, all animalswere killed and the distracted calluseswere harvested and processed for histological, scanning electron microscopic, as well as Ca/P ratio analysis.Results The regenerated bone was found in the distraction gap after mandibular lengthening. The mandibular side treated with rhBMP-2 had greater amounts of newbone formation and earliermineralization than contra-lateral side (non-rhBMP-2 treated).Conclusion Recombinant human BMP-2 appears to be able to accelerate bone formation of mandibular distraction osteogenesis in rabbits.
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