硫代反义Bcl-xL寡脱氧核苷酸对BcaCD885颊癌细胞凋亡及热敏感性的影响
Effect of Phosphorothioate Antisense Bcl-xL Oligodeoxynucleotides on Apoptosis and Thermosensitivity of BcaCD885 Cells
作者：何永文,边莉,梁新华
Author：HE Yong-wen1,BIANLi2,LIANG Xin-hua3
收稿日期：2004-04-25 年卷（期）页码：2004,22(02):112-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：反义寡脱氧核苷酸,Bcl-xL,BcaCD885细胞,凋亡,热敏感性,
Key words：antisense oligodeoxynucleotides,Bcl-xL,BcaCD885 cells,apoptosis,thermosensitivity,
基金项目：云南省教育厅科学研究基金资助项目(0112444)
中文摘要
目的 探讨硫代反义Bcl-xL寡脱氧核苷酸(AS-sODN)对BcaCD885颊癌细胞凋亡及热敏感性的影响。方法 以脂质体Lipofectin为载体,转染AS-sODN于BcaCD885细胞内,倒置显微镜观察细胞形态学改变,TUNEL原位末端标记检测细胞凋亡,流式细胞技术(FCM)测定细胞凋亡率及Bcl-xL蛋白表达水平;转染AS-sODN于BcaCD885 细胞内,再以43℃,40 min加热细胞,FCM检测细胞凋亡率。结果 AS-sODN转染后,BcaCD885颊癌细胞出现皱缩、呈浮起状,TUNEL原位末端标记阳性,Bcl-xL蛋白表达明显下降(P
英文摘要
Objective To studythe effect of phosphorothioate antisense Bcl-xLoligodeoxynucleotides on apoptosis and thermo- sensitivity of BcaCD885 cells.Methods After phosphorothioate antisense Bcl-xL oligodeoxynucleotides were tranfected into BcaCD885 cells. The characteristics of apoptotic cellswere evaluated bymorphological observation andTUNELstaining. Apoptotic rate and Bcl-xL protein expression were analyzed with flow cytometry. The influence of phosphorothioate antisense Bcl-xL oligode- oxynucleotides on apoptotic rate of BcaCD885 cells induced by hyperthermiawith 43℃40 minwas also examined through flow cy- tometry.Results The BcaCD885 cells transfected with phosphorothioate antisense Bcl-xL oligodeoxynucleotides displayed apop- totic morphological features. The Bcl-xL protein expression level of these cells was down-regulated significantly compared with the controlled group (P
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