颞下颌关节盘前移位后髁突软骨细胞基质基因表达的变化
Gene Expression Changes of Cartilage Matrix of Condylar Cartilage with Anterior Disc Displacement
作者：冯剑颖,张凯,谷志远,柴田·考典
Author：FENGJian-ying1, ZHANGKai1,GUZhi-yuan1,SHIBATATakanori2
收稿日期：2004-04-25 年卷（期）页码：2004,22(02):100-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：颞下颌关节,关节盘前移位,Ⅱ型胶原,蛋白多糖聚合体,原位杂交,
Key words：temporomandibular joint,disc displacement,collagenⅡ,aggrecan,in situhybridization,
基金项目：
浙江省科技计划项目(2003C33024)及卫生部口腔计算机工程技术应用研究中心项目(G20030241)
中文摘要
目的 探讨颞下颌关节盘前移位后髁突软骨细胞基质基因表达的变化。方法 建立40只大白兔颞下颌关节盘前移位动物模型,用地高辛标记cRNA探针原位杂交技术,检测术后不同病变时期髁突软骨细胞Ⅱ型胶原和蛋白多糖聚合体基因表达的改变。结果 正常髁突的增殖层深层、肥大层和钙化层细胞胞浆内可见大量Ⅱ型胶原和蛋白多糖聚合体mRNA表达。术后1周蛋白多糖聚合体开始下调,至2周达低谷,6周后恢复至正常水平。Ⅱ型胶原4周后开始持续下降,其表达下降程度较蛋白多糖聚合体显著,8周后逐渐恢复正常。结论 关节盘前移位后髁突软骨的基质基因表达发生有序、协调的变化,这种变化意味着适应性改建的启动。
英文摘要
Objective To study the gene expression changes in extracelluar matrix of condylar cartilage following disc anterior displacement of rabbit TMJ.Methods The right sides of 28 joints in 40 rabbits were subjected to surgical operation of disc dis- placement. The condylar CollagenⅡand Aggrecan mRNA expression were detected byin situhybridization.Results Collagen II and Aggrecan mRNAmainly expressed in the lower zone of condylar chondrocyte. Aggrecan mRNA decreased faster than colla- gen II following disc displacement, and adjusted to normal later.Conclusion Anterior disc displacement leads to alteration of ex- tracellurar matrix gene expression in the condylar chondrocyte, which means the start of remodeling.
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