Objective To study the correlation of prime colonization time ofMutans Streptococciand feeding habits in infants. Methods One hundred and eighty children(aged 6~24 months) from Shenyang city were examined for the colonization ofMS Related items were registered by completed questionnaires.Results The study showed a correlation between prime colonization time and feeding mode, breast feeding, feeding frequency during bedtime, asleep habits.Conclusion Advocating reasonable feeding mode and asleep habits is effective to interdict or delayMS′s colonization and transmission in child to prevent caries.