氨基胍干预对创伤性面瘫大鼠外周面神经及周围组织一氧化氮合酶表达的影响
Effects of Aminoguanidine on the Expression of NOS in Facial Nerve and Surrounding Tissues of Traumatic Facial Paralysis Rats
作者：王立军1,周树夏2,顾晓明1,张 英1
Author：WANG Li-jun1,ZHOU Shu-xia2,GUXiao-ming1,ZHANG Ying1
收稿日期：2004-02-25 年卷（期）页码：2004,22(01):7-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：氨基胍,一氧化氮合酶,面瘫,面神经,免疫组织化学,大鼠,
Key words：aminoguanidine,nitric oxide synthase,facial paralysis,facial nerve,immunohistochemistry,rat,
基金项目：全军指令性课题(96L047)
中文摘要
目的 研究诱导型一氧化氮合酶抑制剂氨基胍对创伤性面瘫大鼠外周面神经及周围组织一氧化氮合酶表达的影响。方法 通过大鼠面瘫前后腹膜内小剂量给予氨基胍,在伤后各个时间点切取损伤面神经和软组织, 采用兔抗大鼠一氧化氮合酶(NOS)抗血清免疫组织化学ABC法对面神经和肌肉软组织内NOS表达的变化进行研究。结果 氨基胍组面神经和肌肉软组织伤后诱导型一氧化氮合酶免疫反应性明显降低。结论 氨基胍慢性干预明显抑制面神经和肌肉软组织内诱导型一氧化氮合酶的表达,为面神经再生与组织创伤修复创造了有利条件。
英文摘要
Objective To study the effects of inducible NOS inhibitor aminoguanidine on the expression of NOS in facial nerve and surrounding tissue of traumatic facial paralysis rats.Methods Asmall dose of aminoguanidine were intraperitoneally injected into rats before and after facial paralysis. The facial nerve and surrounding tissues were cut at different time point. Immunohisto- chemical ABC method was used to study the changes of NOS expression in facial nerve and surrounding tissues.Results The in- ducible NOS immunoreactivity was obvious inhibited in the facial nerve and surrounding tissues in aminoguanidine group.Conclu- sion Aminoguanidine chronic treatment can obvious inhibit the inducible NOS expression in the facial nerve and surrounding tis- sues. Aminoguanidine can improve the regeneration of facial nerve and the recovery of traumatic tissues.
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