Notch_2-Delta在体外人牙髓细胞分化中的表达及调控
The Roles of Notch2-Delta Signaling in the Differentiation of Longterm Cultured Human Dental Pulp Cells
作者：何飞,谭颖徽,杨峥嵘
Author：HE Fei*,TANYinghui,YANGZhengrong.
收稿日期：2003-10-25 年卷（期）页码：2003,21(05):344-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：信号转导,牙髓细胞,细胞培养,Notch,Delta,
Key words：signaling reduction,pulp cell,cell culture,Notch,Delta,
基金项目：本课题为全军医药卫生科研基金资助项目(编号01MA165)
中文摘要
目的 探讨Notch信号在体外牙髓细胞向成牙本质样细胞分化过程中的作用。方法 人牙髓细胞体外连续培养,采用免疫组化染色及Western blot方法对该过程中人牙髓细胞Notch2-Delta的表达及rhBMP-2对Delta 表达的影响进行定性及半定量研究。结果 体外牙髓细胞增殖、分化过程中有Notch2-Delta的表达,随细胞增殖、分化状态的不同,二者表达的部位及表达强度发生变化,外源性rhBMP-2在牙髓细胞结节形成后期可上调Delta的表达。结论 Notch信号途径参与了体外牙髓细胞向成牙本质样细胞分化过程,其介导的细胞间相互作用可能是控制牙髓细胞对信号分子反应性的机制。
英文摘要
Objective To studythe roles of the Notch2-Delta signaling in the differentiation of dental pulp cells into odon- toblastsin vitro.Methods A long-term culture system was used as a model for the study of proliferation and differentiation of pulp cells into preodontoblasts. The expression patterns ofNotch2and its ligand Delta in different phases of long-term cultured den- tal pulp cellswere studied by immunohistochemistry andwestern blotting. Furthermore, the effects of rhBMP-2 on the expression of Notch signaling members were studied.Results Notch2and its ligand Delta expressed in all phases of dental pulp cells and the sites and levels of their expression changed with different phases. rhBMP-2 could significantly up-regulated the expression of Delta in the phase of later cell nodule formation.Conclusion This study demonstrated that Notch signaling pathway played roles in the differentiation of long-term cultured human dental pulp cells which formed nudules that were slightly mineralized and had ul- trastructural features reminiscient of preodontoblasts, and it may be the mechanism that control pulpal cell’s responsiveness to sig- naling molecules in the healing of wound pulp.
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