组织工程化口腔黏膜移植修复裸露硬腭对上颌骨生长发育影响的研究
Maxillary Growth Following Tissue Engineered Oral Mucosal Implantation on Mucoperiosteal Denudated Palate Process in Young Rat
作者：石冰,宋庆高,黄旭,李盛,鲁勇,杨维东
Author：SHI Bing*,SONG Qinggao,HUANGXu,et al.
收稿日期：2003-08-25 年卷（期）页码：2003,21(04):255-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：腭裂,组织工程化口腔黏膜,黏膜移植,骨面裸露,生长发育,
Key words：cleft palate,cultured oral epithelium,mucosal transplantation,denuded bone,maxillary growth,
基金项目：本课题为霍英东基金资助项目(编号71040)
中文摘要
目的 将构建的口腔黏膜异体移植修复腭裂术后遗留的裸露硬腭处,观察其对腭裂术后上颌骨继发畸形的防治效果。方法 分离提取SD乳鼠硬腭黏膜细胞并对其进行培养,以自制藻酸钠薄膜作为支架材料构建组织工程化的口腔黏膜。3周龄雌性SD大鼠,随机分成1个正常对照组和3个实验组,每组20只。正常对照组无任何处理,实验组均行左侧硬腭黏膜骨膜瓣切除,Ⅰ组切除后任其裸露(裸露组),Ⅱ组、Ⅲ组分别行藻酸钠薄膜修复(材料组)和组织工程化的口腔黏膜修复(黏膜组)。术后9周(12周龄)处死动物,体视显微镜下测量左右侧硬腭骨宽度,比较各组的非对称率。结果 裸露组与材料组左右侧硬腭骨宽度非对称率无差异;黏膜组非对称率小于裸露组和材料组。结论 组织工程化口腔黏膜移植修复裸露硬腭对腭裂术后上颌骨继发畸形有一定的预防效果。
英文摘要
Objective To observe the effectiveness of prevention and cure for maxillary growth deformity following tissue engineered oral mucosa implantation on mucoperiosteal denuded palate process in young rat.Methods Hard palate mucoperios- teum of a SD baby ratwere excised and oral keratinocyteswere isolated and cultured. Tissue engineered oral mucosawas fabricated with the cultured oral keratinocytes and the membrane made of sodium alginate (SA). 80 female three-week-old SD ratswere used as subjects in this study. The animals were divided randomly into a normal control group and 3 experimental groups, each group included 20 rats. Normal control group (NG) were not operated. Hard palate mucoperiosteum on left side in all experimental groups were excised, exposed bonewere nottreated in denuded group (DG), but repairedwithmembrane inmaterial group (MG) and repaired with the tissue engineered oral mucosa in mucosal group (MUG). All the animalswere sacrificed at 9thweek postop- eratively (12 weeks old), and the clean widths of right and left hard palatal were measured under a dissection microscope. The difference between palatal widths of two sides and the asymmetry ratio between the different groups were compared and analyzed. Results No significant difference in asymmetrywas discovered between the DG and theMG, butthe asymmetry inMUGwas less than DG orMG.Conclusion Tissue engineered oral mucosal implantation in palatoplasty is an effective method in preventing and curing secondary maxilla deformity by repairing denuded bone wound.
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