下颌骨骨折愈合过程中整合素α_5表达的免疫组化研究
Immunohistochemical Research of Integrinα5Expression during Mandibular Fracture Healing
作者：刘少华,李声伟,程刚,田卫东,刘磊,魏世成,陈伟辉
Author：LIUShaohua*,LIShengwei,CHENG Gang,et al.
收稿日期：2003-04-25 年卷（期）页码：2003,21(02):101-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：整合素α5,下颌骨,骨折愈合,免疫组织化学,
Key words：integrinα5,mandible,fracture healing,immunohistochemistry,
基金项目：
中文摘要
目的 探讨下颌骨骨折愈合过程中整合素α5表达水平的改变。方法 用兔作下颌骨骨折的动物模型,分别于骨折前1天及骨折后第1、3、5、7、14、30、60、90天取骨断端组织标本作成石蜡切片并用LsAB法进行免疫组织化学实验,以探讨整合素α5在骨组织,特别是在成骨细胞和破骨细胞中的表达。结果 整合素α5在骨组织及其周围软组织中广泛表达;从骨折后第7天出现增强,第14天到第30天表达最强,第60~第90天后接近正常。结论 在下颌骨骨折的愈合过程中,整合素α5在骨组织细胞上的表达增强,整合素α5对骨折的愈合过程具有重要作用。
英文摘要
Objective To investigate the expression change of integrinα5during mandibular fracture healing.Methods Using rabbit mandibular fracture model, the fractured bone tissues were obtained and paraffin slices were made on the days of 1, 3,5,7,14,30,60 and 90 after surgery, respectively. Non-fractured mandibles were used as normal control. LsAB immunohisto- chemical methodwas used to detectthe expression of integrinα5in bone tissue, especially on osteoblasts and osteoclasts.Results Integrinα5was widely expressed in fractured bone and surrounding soft tissues. The expression of integrinα5increased 7 days after fracture, peaked in 14 to 30 days and returned to nearly normal in 60-90 days.Conclusion During mandibular fracture healing, the expression of integrinα5in bone tissue increased clearly. It is estimated that integrinα5playes an important role in fracture healing.
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