碱性成纤维细胞生长因子和纤维连接蛋白在大鼠组织工程复合皮肤愈合中的表达
Expression of Basic Fibroblast Growth Factor and Fibronectin in Tissue Engineering Skin Allograft During Healing Process
作者：王军琳,金岩,郭征,刘晓亮,刘源,赵宇,胡静
Author：WANGJunlin*,JIN Yan,GUO Zheng,et al.
收稿日期：2003-02-25 年卷（期）页码：2003,21(01):41-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：皮肤,碱性成纤维细胞生长因子,纤维连接蛋白,移植,
Key words：skin,basic fibroblast growth factor,fibronection,allograft,
基金项目：
中文摘要
目的 探讨碱性成纤维细胞生长因子(bFGF)和纤维连接蛋白(FN)在组织工程皮肤愈合过程中的作用。方法 以新生SD大鼠皮肤为原材料培养组织工程复合皮肤并将其移植到15只成年SD大鼠,第7、10、14、20、30天取标本,通过HE染色、免疫组织化学方法对移植后不同时间的组织工程复合皮肤进行检测,观察创面愈合过程中bFGF、 FN的表达变化及其与组织修复的关系。以做自体皮肤移植和正常Wistar大鼠做对照。结果 术后第10天时bFGF、 FN表达明显,术后第10天以前和第14天以后表达较弱。bFGF、FN在组织工程皮肤愈合过程中的变化与自体皮肤移植愈合过程相似。结论 在组织工程皮肤移植修复大鼠皮肤缺损中bFGF、FN有表达,促进了创伤愈合。
英文摘要
Objective The aim of this study was to explore the expression of basic fibroblast growth factor (bFGF) and fi- bronectin during the healing process of allografttissue engineering skin.Methods The tissue engineering skin thatwas obtained from neonatal SD rats was cultured in the lab. Afterwards, the skin was grafted into adult SD rats, and the expressions of bFGF and fi- bronectin were detected on the 7th, 10th,14th, 20th and 30th day after the allograft of the tissue engineering skin. The autografted skin in 15 adult Wistar rats and the normal skin in 15 Wistarwere treated as the control. HE staining and immunohistochemical stain- ing were used to examine the healing of grafted skin.Results The expression of bFGF and fibronectin was the strongest on the 10th day aftergraft, andwasweaker before the 10th day and afterthe 14th day. The expression changes of bFGF and fibronectinwere sim- ilar as they were in the autograft group.Conclusion The expression changes of bFGF and fibronectin in the tissue engineering skin during the process of wound healing were similar to those of autografted skin, and these can promote the repair of tissue engineering skin allograft.
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