人白细胞介素1受体拮抗剂基因转染人胚颞颌关节软骨细胞的实验研究
An Experimental Study on Gene Transfection of Human Interleukin-1 Receptor Antagonist Gene into Chondrocytes of Temporomandibular Joint
作者：李逸松,田卫东,王栋,李声伟,陈希哲
Author：LI Yisong,TIANWeidong,WANG Dong,et al.
收稿日期：2003-02-25 年卷（期）页码：2003,21(01):19-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：软骨细胞,人白细胞介素1受体拮抗剂,颞颌关节,骨关节病,基因转染,
Key words：chondrocyte,human interleukin-1 receptor antagonist,temporomandibular joint,osteoarthrosis,gene transfection,
基金项目：
本课题为国家教育部回国人员启动基金(编号363)及四川省青年基金(编号387)资助项目
中文摘要
目的 研究人白细胞介素1受体拮抗剂(hIL-1ra)基因转染人胚颞颌关节软骨细胞的方法和表达。方法 分离培养人胚颞颌关节软骨细胞,将hIL-1ra基因以阳离子脂质体Lipefectine介导进行基因转染,免疫细胞化学染色和ELISA法检测转染细胞中hIL-1ra蛋白的表达。结果 使用阳离子脂质体介导hIL-1ra基因转染人胚颞颌关节软骨细胞,免疫细胞化学染色hIL-1ra蛋白呈阳性表达,瞬时转染和稳定转染时,转染细胞胞浆内和培养基上清液中均有目的基因的表达,与未经转染的对照组存在显著性差异(P
英文摘要
Objective The purpose of this study was to investigate methods and expression of the recombinant human inter- leukin-1 receptor antagonist gene transfected into chondrocytes of temporomandibular joints (TMJ).Methods Chondrocytes derived from the TMJ of 5~7 months old human fetus were transfected by recombinant human interleukin-1 receptor antagonist gene via cat- ionic liposome (Lipefectine) as a medium. The expressing level of hIL-1ra protein was detected using immunocytochemistry and enz- yme-linked immunosorbent assay (ELISA).Results The positive results of the immunocytochemistry were found. The positive ex- pression was detected in the cell plasma and the cell culture supernatant, and there was significant difference between the cells with and without gene transfection (P
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