胶原蛋白在咬肌附着及再附着界面的表达
Expression of Collagens in Reattached Masseter Muscles to Mandibles Following a Surgical Detachment
作者：季彤,张陈平,解雪涛
Author：JITong,ZHANG Chenping,XIEXuetao.
收稿日期：2003-02-25 年卷（期）页码：2003,21(01):16-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：咬肌,下颌骨,再附着,界面,免疫组织化学,
Key words：masseter muscle,mandible reattachment interface,immunohistochemistry,
基金项目：本课题为上海市卫生局百人计划资助(编号97BR015)
中文摘要
目的 探讨山羊咬肌与下颌骨皮质骨面和松质骨面再附着中胶原在肌—骨界面的表达及意义。方法 以9只成年山羊为研究对象,其中1只为正常对照。将实验组山羊双侧下颌骨咬肌附着从骨面剥离,附着区骨面平均分为二部分,分别保留原骨面或去除皮质骨,暴露松质骨,咬肌原位缝合。术后2、4、8、12周采集标本,采用免疫组织化学ABC法检测两种肌—骨界面Ⅰ、Ⅱ、Ⅲ型胶原的表达情况。结果 免疫组化观察表明,Ⅰ、Ⅱ、Ⅲ型胶原在愈合过程中的表达不同,但两种骨面的肌—骨界面胶原表达无明显差别。结论 再附着界面胶原表达随时间变化而与附着骨面结构无关。
英文摘要
Objective The aim of this study was to investigate the expression of collagen in the process of masseter muscle reattachment to the cortical and cancellous bones ofmandible.Methods Atotal of nine adult goatswere used in the study. Onewas the control. The other eight were treated with bilateral detachment of the masseter muscles. The biopsies of bone and muscle were taken at 2, 4, 8 and 12 weeks after the operation. The characteristics of the healing muscle-bone interfaceswere examined using im- munohistochemical techniques.Results Immunohistochemical analysis illustrated that the locations of collagen typeⅠ,ⅡandⅢ were different during the healing process, but similar in the cortical and cancellous bones.Conclusion This study demonstrates that the distribution of the three types of collagens at the muscle-bone interfaces is associated with time, but not related with their loca- tions.
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