纤维粘连蛋白在牙髓修复中作用的免疫组织化学研究
Roles of Fibronectin in Reparative Dentinogenesis: An Immunohistochemical Study
作者：陈智,樊明文,吕平,边专,张旗
Author：Chen Zhi, FanMingwen, L?Ping, et al
收稿日期：2002-04-25 年卷（期）页码：2002,20(02):104-
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：修复性牙本质,纤维粘连蛋白,牙髓,免疫组织化学,
Key words：reparative dentin,fibronectin,dental pulp,immunohistochemistry,
基金项目：
本课题为国家自然科学基金(编号39830430)和湖北省自然科学基金资助项目(编号98J096)
中文摘要
目的:研究纤维粘连蛋白在牙髓修复中的免疫定位和分布特征。方法:在大鼠第一磨牙制备单面洞,分别观察3 d、15 d和30 d后修复性牙本质形成情况。免疫组织化学技术检测纤维粘连蛋白的免疫反应。结果:术后3 d, 修复性牙本质尚未形成。牙髓内成牙本质细胞呈弱阳性染色,前期牙本质也为弱阳性。术后15 d,可见修复性牙本质形成,成牙本质细胞样细胞和牙髓成纤维细胞免疫染色呈阳性。术后30 d,已形成的修复性牙本质内纤维粘连蛋白染色为强阳性,特别集中于修复性牙本质与牙髓的交界面,成牙本质细胞样细胞染色呈强阳性。结论:纤维粘连蛋白可能在修复性牙本质形成中起重要调节作用。
英文摘要
Objective: The aim of this studywas to investigate roles of fibronectin in dental pulp injury and repair.Methods: The cavity was prepared in the mesial surface of the firstmolars ofWistar rats. The ratswere sacrificed in 3, 15, and 30 days post-operation. After histological process, the paraffin sectionswere reactedwithmonoclonal antibodies against ratfibronectin usingSABCmethod. Results: Immunohistochemical stain demonstrated that the positive staining of fibronectin presented in different stages of dental pulp repair. In the group of 3 days, the weak stainingwas located in odontoblasts. After15 days, odontoblast-like cells and dental pulp cells were stained positively. 30 days after operation, the stronger stainingwas observed in odontoblast-like cells and pulp fi- broblasts. Positive staining was seen in reparative dentin, particularly along the interface of reparative dentin and dental pulp. Conclusion: These results demonstrated that fibronectin might play a role of mediation during the reparative dentinogenesis.
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