程序性死亡配体1负性调控慢性牙周炎的炎症反应
Programmed death ligand 1 negatively regulates inflammatory response of chronic periodontitis
作者：袁雯雯 王晓茜 张杰华 周闻捷 冯云 陈娇 张平
Author：Yuan Wenwen, Wang Xiaoqian, Zhang Jiehua, Zhou Wenjie, Feng Yun, Chen Jiao, Zhang Ping
收稿日期： 年卷（期）页码：2015,33(4):366-366-369
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：程序性死亡配体1,慢性牙周炎,免疫调控,牙槽骨吸收,
Key words：programmed death ligand 1, chronic periodontitis,immunoregulation,alveolar absorption,
基金项目：
国家自然科学基金资助项目（81372892）；四川省科技厅基金资助项目（JCPT 2011-9）
中文摘要
目的 探讨程序性死亡配体1（PD-L1）在慢性牙周炎中是否有表达，以及与不同程度慢性牙周炎的相关关系，为阐明慢性牙周炎的免疫调控机制、临床治疗和预后提供依据。方法 收集健康人和慢性牙周炎患者的牙龈和牙周膜组织。根据临床探测，分为正常对照组、轻度牙周炎组、重度牙周炎组。运用荧光定量聚合酶链反应（PCR）测定不同组牙周组织中PD-L1 mRNA的表达；免疫印迹（Western blot）以及免疫组织化学方法测定不同组牙周组织中PD-L1蛋白的表达。结合临床影像资料，分析PD-L1的差异表达量和不同程度慢性牙周炎的关系。结果 轻度牙周炎组牙周组织中PD-L1的相对表达量显著高于重度牙周炎组（P0.05）。结论 PD-L1在牙周组织的表达可负性调控炎症牙周组织损伤。
英文摘要
ObjectiveTo investigate whether programmed death ligand 1 (PD-L1) expressed in the periodontal tissue of chronic periodontitis and the correlativity of PD-L1 and different degrees of chronic periodontitis, provide experience for immunoregulation mechanism, clinical treatment and prognosis of chronic periodontitis.MethodsGingiva and periodontal tissue of healthy people and chronic periodontitis patients were collected. Based on clinical probing, periodontal tissue were classified into three groups: periodontal tissues of healthy people, periodontal tissue of mild chronic periodontitis, periodontal tissue of severe chronic periodontitis. Fluorescent quantitation polymerase chain reaction was applied to explore the expression of PD-L1 mRNA in the periodontal tissue of the different groups. Western blot and immunohistochemistry method were utilized to test the expression of PD-L1 protein in the periodontal tissue of the different groups. Combining with clinical image data, the relationship between differentially expressions of PD-L1 and different degrees of chronic periodontitis was analyzed.ResultsThe relative expression quantity of PD-L1 in the periodontal tissue of the mild chronic periodontitis was significantly higher that of the severe chronic periodontitis (P0.05).ConclusionThe expression of PD-L1 in the periodontal tissue negatively regulates inflammatory periodontal tissue damage.
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