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论文摘要

不同频率微振动对血管内皮细胞生长因子表达及通透性的影响

Effects of different frequency microvibrations in the vascular endothelial growth factor expression and permeability of vascular endothelial cell

作者:朱卓立 马瑞阳 杨扬 甘雪琦

Author:Zhu Zhuoli, Ma Ruiyang, Yang Yang, Gan Xueqi.

收稿日期:2015-08-03          年卷(期)页码:2016,34(2):136-136-139

期刊名称:华西口腔医学杂志

Journal Name:West China Journal of Stomatology

关键字:低频微振动,内皮细胞,血管内皮细胞生长因子,通透性,

Key words:low frequency microvibration,endothelial cell,vascular endothelial growth factor,permeability,

基金项目:

高等学校博士学科点专项科研基金(20120181120007)

中文摘要

目的 研究微振动对血管内皮细胞分泌血管内皮细胞生长因子(VEGF)及通透性的影响。方法 培养人脐静脉血管内皮细胞(HUVEC),每日施加0.2、0.5、2、5 Hz 4种低频率微振动30 min,通过Tagman探针实时荧光定量聚合酶链反应(PCR)及蛋白质印迹法检测VEGF的表达,并检测内皮细胞的通透性。结果 加载0.2 Hz和0.5 Hz微振动可使血管内皮细胞VEGF mRNA及蛋白表达上调(PPPP结论 0.2~0.5 Hz的微振动可使血管内皮细胞分泌VEGF增高,并提高血管内皮细胞的通透性。

英文摘要

ObjectiveThis study aimed to evaluate the vascular endothelial growth factor (VEGF) expression and permeability of vascular endothelial cell under microvibration.Methods Human umbilical vein endothelial cell (HUVEC) were cultured, randomly vibrated under low frequency of 0.2, 0.5, 2, 5 Hz, 30 min per day. The VEGF mRNA level was detected by Tagman probe real-time fluorescence quantitative polymerase chain reaction (PCR), and the VEGF protein expression level was detected by Western blot. The permeability of vascular endothelial cell was evaluated.Results Compared with the blank control group, the mRNA and protein expression level of VEGF were significantly increased under 0.2, 0.5 Hz microvibration (PPPPConclusion0.2-0.5 Hz microvibration can up-regulate the expression of VEGF mRNA and protein in vascular endo- thelial, and increase the permeability.

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