变异链球菌磷酸转移酶系统ptxA、ptxB基因对细菌生长能力的影响
Effect of ptxA and ptxB genes of phosphotransferase system on growth of Streptococcus mutans
作者：吴昕彧,陈晓丹,赵望泓,侯晋,陈璇
Author：Wu Xinyu, Chen Xiaodan, Zhao Wanghong, Hou Jin, Chen Xuan
收稿日期：2015-08-11 年卷（期）页码：2016,34(3):302-302-306
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：变异链球菌,磷酸转移酶系统,ptxA基因,ptxB基因,抗坏血酸,
Key words：Streptococcus mutans,phosphotransferase system,ptxA gene,ptxB gene,L-ascorbate,
基金项目：国家自然科学基金项目（81050035）；广东省高校人才 引进项目（C1030636）；南方医科大学南方医院院长基金项目（2013B003）
中文摘要
目的 研究变异链球菌（S. mutans）磷酸转移酶系统（PTS）中抗坏血酸家族ptxA、ptxB基因对细菌生长能力的影响。方法 构建ptxA、ptxB和ptxAB双重基因缺陷菌株以及ptxAB功能补偿菌株。在仅以抗坏血酸作为唯一碳源时，使用实时荧光定量聚合酶链反应检测野生株中ptxA、ptxB基因的表达情况。连续监测野生株、缺陷株和补偿株的菌液OD600值，比较其生长能力。结果 经过聚合酶链反应和测序鉴定，结果证明缺陷株和补偿株构建成功。在以抗坏血酸作为唯一碳源的培养基中，野生株ptxA、ptxB基因的表达量均明显增高（PS. mutans对抗坏血酸的吸收利用密切相关，缺陷株和补偿株的构建为进一步研究目的基因在S. mutans抗坏血酸代谢中的作用机制提供了理论基础。
英文摘要
Objective This study aims to evaluate the effect of ptxA and ptxB genes, which are important genes in the L-ascorbate phosphotransferase system (PTS) ofStreptococcus mutans(S. mutans). Methods The ptxA-, ptxB-, and ptxABdouble deficient mutant as well as ptxAB-complemented strain were constructed. Quantitative real-time polymerase chain reaction analysis was performed to evaluate the expression of the target genes of wild-typeS. mutanswhen L-ascorbate was used as the sole carbohydrate source. The OD600values of the wild type, deficient, and complemented strains were continuously monitored, and their growth curves were constructed to compare growth capacity. Results Polymerase chain reaction and sequencing analyses suggested that deficient and complemented strains were successfully constructed. The expression levels of ptxA and ptxB significantly increased (PS. mutans. The construction of deficient strains and complemented strain lay a foundation for further mechanism study on L-ascorbate metabolism inS. mutans.
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