脉冲超声及电磁场对大鼠骨髓间充质干细胞体外成软骨分化过程中细胞外基质溢出的影响
Effects of pulsed ultrasound and pulsed electromagnetic field on the extracellular matrix secretion of rat bone marrow mesenchymal stem cell pellets in chondrogenesis
作者：张智,唐娜,王珏,赵志河,谭理军
Author：Zhang Zhi, Tang Na, Wang Jue, Zhao Zhihe, Tan Lijun
收稿日期：2015-12-16 年卷（期）页码：2016,34(3):291-291-294
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：脉冲超声,脉冲电磁场,成软骨分化,细胞外基质,
Key words：pulsed ultrasound,pulsed electromagnetic field,chondrogenesis,extracellular matrix,
基金项目：国家自然科学基金（30900287，81030034，30900286）
中文摘要
目的 研究脉冲超声以及脉冲电磁场对大鼠骨髓间充质干细胞微球体外成软骨分化中细胞外基质向培养基中溢出的影响。方法 将干细胞微球在成软骨培养基中进行培养，分为无刺激组（N组）、脉冲超声（100、150、200 mW·cm-2）刺激组和脉冲电磁场（1、2、5 mT）刺激组。2周后采用酶联免疫吸附测定法检测培养液中Ⅱ型胶原和糖胺聚糖的质量浓度。结果 脉冲超声刺激组细胞外基质分泌较N组增多（PP>0.05）；脉冲电磁场组对Ⅱ型胶原分泌无明显影响（P>0.05），1 mT组对糖胺聚糖的分泌无明显影响（P>0.05），2 mT和5 mT组糖胺聚糖的分泌减少（P
英文摘要
Objective To study the effects of pulsed ultrasound (PUS) and pulsed electromagnetic fields (PEMF) on the secretion of extracellular matrix from a culture complex duringin vitrochondrogenesis.Methods All the rat bone marrow mesenchymal stem cell pellets were cultured in achondrogenic medium. Different intensities of PUS (100, 150, and 200 mW·cm-2) and PEMF (1, 2, and 5 mT) were applied to the cell pellets for 2 weeks. Group N was cultured without PUS and PEMF stimulation as control. The culture medium was collected after 2 weeks of culture. Enzyme-linked immunosorbent assay (ELISA) was used to detect the type of collagen and glycosaminoglycan (GAG) in the culture medium. Results PUS increased the secreting-type collagen and GAG from cell pellets compared with group N (PP>0.05). PEMF had no significant effect on the secretion of the type of collagen (P>0.05). A PEMF of 1 mT had no significant effect on the secretion of GAG (P>0.05). A PEMF 2 and 5 mT decreased the secretion of GAG (P
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