Objective The effect of treated dentin matrix (TDM) to the proliferation and osteogenesis differentiation of dentine particles suffering from gradient demineralization. Human BMSCs were isolated and cultivated, and subsequently cultivated in the TDM leaching solution. The proliferation of BMSCs was detected by CCK-8. The osteogenesis-related proteins, including collagen typeⅠ(ColⅠ) and runt-related transcription factor-2 (Runx2), were extracted and detected by Western blot after a 7-day culture. Results Compared with the control group and hydroxyapatite (HA)/ β-tricalcium phosphate (β–TCP) group, the proliferation of BMSCs cultivated in TDM leaching solution was significantly improved. The expression of ColⅠ and Runx2 obviously increased after the 7-day cultivation in TDM leaching solution. Conclusion TDM can promote the proliferation and osteogenesis differentiation of BMSCs, implying the feasibility of the application in bone tissue engineering.
