尼古丁调控人牙周膜细胞自噬水平的实验研究

ObjectiveTo explore the effect of nicotine on the autophagy level of human periodontal ligament cells (hPDLCs).MethodsPeriodontal tissues collected from premolars for orthodontic treatment reasons were used to culture hPDLCs. Western blot analysis was performed to test the most optimal time and concentration of nicotine on the autophagy level of the hPDLCs. Transmission electron microscope and immunofluorescence observation were carried out to detect the form of autophagosomes and expression of autophagy related protein LC3 in hPDLCs under this optimal condition.ResultsProtein expression of LC3Ⅱ was up regulated with the 12 h nicotine stimulating. Besides that, the up regulation of the protein expression of LC3Ⅱ was concentration dependent and nicotine with a concentration of 1×10^-5mol·L^-1was the most optimal condition. Transmis-sion electron microscope and immunofluorescence observations indicated that nicotine would activate the autophagy level of hPDLCs by increasing the number of autophagosomes and up regulating the expression of autophagy related protein LC3.ConclusionNicotine could increase autophagy level of hPDLCs, thus affecting the occurrence and development of smoking related periodontitis.