Root canal filling is performed as the final and most important procedure of root canal treatment. The superior 3D filling is the key determinant of endodontic success. However, such procedure remains a challenge because of the comple-xity of the root canal system and the limitation of root canal filling materials and methods. This paper provides an overview of current principles and practices in root canal filling, focusing on advantages, disadvantages and indications. The process errors and countermeasures in various root canal filling methods are also discussed. This review provides guidance and help for clinical and practice to achieve a satisfactory root canal filling and improve root canal treatment outcome.