半乳糖凝集素-3基因在口腔鳞状细胞癌增殖、侵袭、凋亡中的作用及机制研究
Role and mechanism of Galectin-3 gene in proliferation, invasion, and apoptosis of oral squamous cell carcinoma
作者：方政, 邱峰, 赵军方, 孙强, 乔彬, 李光辉, 李新明
Author：Zheng Fang, Feng Qiu, Junfang Zhao, Qiang Sun, Bin Qiao, Guanghui Li, Xinming Li
收稿日期：2017-07-27 年卷（期）页码：2018,36(4):404-404-409
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：半乳糖凝集素-3基因,口腔鳞状细胞癌,增殖,侵袭,凋亡,
Key words：Galectin-3 gene,oral squamous cell car-cinoma,proliferation,invasion,apoptosis,
基金项目：国家自然科学基金（8140100298）
中文摘要
目的 探讨抑制口腔鳞状细胞癌（OSCC）中半乳糖凝集素-3（Galectin-3）基因的表达对癌细胞增殖、侵袭、凋亡的影响及机制。方法 逆转录聚合酶链反应（RT-PCR）和蛋白质印迹（Western blotting）分别检测OSCC中Galectin-3 mRNA和蛋白表达;人OSCC Tca8113分为空白组、阴性对照组和Galectin-3转染组,转染48 h后,Western blotting检测各组细胞中Galectin-3、基质金属蛋白酶2（MMP-2）、基质金属蛋白酶9（MMP-9）、活化的含半胱氨酸的天冬氨酸蛋白水解酶3（Cleaved Caspase-3）、β-连环蛋白（β-catenin）、细胞周期素D1（Cyclin D1）蛋白表达;CCK8检测细胞增殖;Transwell小室检测细胞侵袭能力;流式细胞术检测细胞凋亡。结果 OSCC中Galectin-3 mRNA及蛋白表达均显著高于癌旁组织（P<0.01）;RNA干扰Galectin-3表达后Tca8113细胞中Galectin-3蛋白表达明显降低;Galectin-3转染组细胞存活率、细胞侵袭能力及MMP-2、MMP-9、β-catenin、Cyclin D1蛋白表达均显著低于空白组,细胞凋亡率及Cleaved Caspase-3蛋白表达均显著高于空白组（P<0.01）。结论 抑制OSCC中Galectin-3基因表达可显著降低癌细胞的增殖及侵袭能力,诱导细胞凋亡,其机制与下调Wnt/β-catenin信号通路有关。
英文摘要
ObjectiveThe effect and mechanism of Galectin-3 gene expression on proliferation, invasion, and apoptosis of oral squamous cell carcinoma (OSCC) were investigated.MethodsReverse transcription-polymerase chain reaction (RT-PCR) and Western blotting were used to detect the mRNA and protein of Galectin-3 gene in OSCC. OSCC Tca8113 was divided into control, negative control, and Galectin-3 transfection groups. Western blotting was used to detect the expression of Galectin-3, matrix metalloproteinase (MMP)-2, MMP-9, Cleaved Caspase-3, β-catenin, and Cyclin D1 protein after transfection for 48 h in each group. Cell proliferation was detected by CCK8. Cell invasion ability was detected by using a Transwell chamber. Cell apoptosis was detected by flow cytometry.ResultsThe mRNA and protein expression levels of Galectin-3 gene in OSCC were significantly higher than those in adjacent tissues (P<0 .01). galectin-3 protein expression in tca8113 cells significantly decreased after rna interference. cell survival rate and invasion as well as mmp-2, mmp-9, β-catenin, and cyclin d1 protein expression were significantly lower than the blank group. apoptosis rate and cleaved caspase-3 protein expression were sig-nificantly higher than the control group (P<0 .01).ConclusionInhibition of Galectin-3 gene expression in OSCC can signi-ficantly reduce the proliferation and invasion of cancer cells and induce apoptosis. The mechanism is related to downregulation of the Wnt/β-catenin signaling pathway.
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