sortase A与变异链球菌致龋性关系的代谢组学研究
Link between sortase A function and cariogenicity of Streptococcus mutans: a preliminary metabolomics analysis
作者：宋颖, 周京琳, 何远丽, 李伟, 邹玲
Author：Ying Song, Jinglin Zhou, Yuanli He, Wei Li, Ling Zou
收稿日期：2017-12-11 年卷（期）页码：2018,36(4):360-360-366
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：代谢组学,变异链球菌,sortase A,<sup>1</sup>H核磁共振波谱,主成分分析,正交偏最小二乘法—判别分析;,
Key words：metabonomics,Streptococcus mutans,sortase A,1H nuclear magnetic resonance spectroscopy,princi-pal component analysis,orthogonality partial least squares discrimination analysis,
基金项目：国家自然科学基金（81570974）;四川省科技厅基金（20-15JY0260）
中文摘要
目的 探索srtA基因编码的转肽酶sortase A（SrtA）影响变异链球菌致龋性的机制。方法 采用基于1H核磁共振波谱（NMR）的代谢组学方法,比较野生型变异链球菌UA159与srtA基因缺陷型变异链球菌UA159的胞外代谢产物;联合使用主成分分析和正交偏最小二乘法—判别分析鉴定不同菌株之间代谢产物的差异。结果 大多数有差异性的代谢产物是未知的,可识别的差异性代谢产物有亮氨酸和缬氨酸（δ 0.92×10-6~1.20×10-6）、乳酸（δ 1.28×10-6）、酮戊二酸（δ 3.00×10-6）、甘氨酸（δ 3.60×10-6）。结论 在进一步完善微生物代谢产物数据库的基础上,1H NMR代谢组学可用于龋病病因的研究。
英文摘要
ObjectiveThis study intends to explore the mechanism underlying the support of sortase A (SrtA) of the cario-genicity ofStreptococcus mutans(S. mutans).MethodsWe performed a metabonomics study based on1H nuclear magnetic resonance spectroscopy (NMR), in which we compared the extracellular metabolites of wild-typeS. mutansUA159 with those of its SrtA-deficient strain. Metabolite differences among strains were identified using a combination of principal component analysis and orthogonality partial least square discriminant analysis.ResultsSeveral differences corresponding mostly to unknown metabolites were identified. Some amino acids such as leucine and valine (δ 0.92×10-6-1.20×10-6), lactic acid ( δ1.28×10-6), oxoglutaric acid (δ 3.00×10-6), and glycine (δ 3.60×10-6) differed among strains.ConclusionThis work establishes the feasibility of using1H NMR-based metabonomics to provide leads for research into molecular factors that promote caries. The database of microbial metabolites should be also improved in further studies.
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