辅助性T17细胞在牙周炎小鼠中的免疫状态研究
Study on the immune status of T helper 17 cells in mice with periodontitis
作者：王琳源 关宁 林晓萍
Author：Wang Linyuan, Guan Ning, Lin Xiaoping.
收稿日期：2014-08-12 年卷（期）页码：2015,33(2):125-125-129
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：舌, 淋巴管, 淋巴引流, 初级淋巴结,辅助性T17细胞,牙龈卟啉单胞菌,牙周炎,白细胞介素-17A,骨吸收,
Key words：tongue , lymphatic vessel , lymphatic drainage , sentinel lymph node,T helper 17 cells,Porphyromonas gingivalis,periodontitis,interleukin-17A,bone resorption,
基金项目：
高等学校博士学科点专项科研基金资助项目（20112104110013）；辽宁省科学技术计划基金资助项目（2012225015）
中文摘要
目的 研究辅助性T（Th）17细胞在牙周炎小鼠中的免疫状态。方法 将7周龄C57BL/6雌性小鼠随机分为牙周炎组和对照组，每组4只。牙周炎组采用口腔涂抹牙龈卟啉单胞菌（P. gingivalis）的方法建立牙周炎动物模型。对照组涂抹PBS液。在涂抹结束后的第4周取材，流式细胞仪检测CD4+维甲酸相关核孤儿受体（ROR）γτ+（Th17）细胞；酶联免疫吸附（ELISA）检测Th17细胞相关的细胞因子白细胞介素（IL）-17A的蛋白表达。结果 牙周炎小鼠牙龈组织、颈部淋巴结和外周血中CD4+ RORγτ+（Th17）细胞在总CD4+ T细胞中的比例和细胞数量显著高于对照组（P结论 在牙周炎的发生发展中，Th17细胞介导的细胞免疫应答增强，牙龈组织、颈部淋巴结和外周血可能是Th17细胞介导免疫应答的主要场所。
英文摘要
ObjectiveThis study investigates the immune status of T helper (Th) 17 cells in mice with periodontitis.MethodsSeven-week-old C57BL/6 female mice were randomly divided into the control and periodontitis groups. Each group comprisedfour mice. Experimental periodontitis was induced in mice by oral infection with Porphyromonas gingivalis in the periodontitis group. Phosphate-buffered saline solution was used in the control group. All mice were sacrificed 4 weeks after the last P. gingivalis infection. CD4+ retinoid-related orphan receptor (ROR) γτ+ (Th17) cells were analyzed by flow cytometry. The protein expression of Th17 cell-related cytokine interleukin (IL)-17A was detected by enzyme-linked immunosorbentassay (ELISA).ResultsFlow cytometry showed that the percentage of CD4+ RORγτ+ (Th17) cells in all CD4+T cells and the cell number in gingival tissues, cervical lymph nodes (CLNs), and the peripheral blood obviously increased (PConclusionTh17 cell-mediated cell response is enhanced, and the gingival tissues, CLNs, and the peripheral blood are probably the main locations of Th17 cell-mediated cell response during the development of periodontitis.
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