下调性别决定区Y框蛋白9对口腔鳞癌细胞上皮间质转化及克隆能力的影响
Effect of down-regulation of sex determining region Y-box 9 on epithelial mesenchymal transition and cloning of oral squamous carcinoma cells
作者：杨文丽,孙明磊,张朋,余炜伟,周海霞,孙强
Author：Wenli Yang,Minglei Sun,Peng Zhang,Weiwei Yu,Haixia Zhou,Qiang Sun
收稿日期：2018-02-18 年卷（期）页码：2019,37(1):13-13-18
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：性别决定区Y框蛋白9,口腔鳞癌,上皮间质转化,细胞克隆,
Key words：sex determining region Y-box 9,oral squa-mous cell carcinoma,epithelial mesenchymal transition,cell clones,
基金项目：河南省教育厅科学技术研究重点项目(14A320022)
中文摘要
目的 探讨下调性别决定区Y框蛋白9（SOX9）对口腔鳞癌（OSCC）细胞上皮间质转化（EMT）及克隆能力的影响。方法 OSCC BcaCD885细胞中转染siRNA control、SOX9 siRNA,同时以不做任何转染的细胞作为control组。实时定量聚合酶链式反应（qRT-PCR）和Western blot筛选干扰效果较好的SOX9 siRNA1做后续研究。细胞克隆实验测定细胞克隆形成能力,免疫荧光检测上皮钙黏附素（E-cadherin）、波形蛋白（Vimentin）表达,Western blot检测E-cadherin、基质金属蛋白酶-2（MMP-2）、Vimentin、基质金属蛋白酶-9（MMP-9）表达,Transwell小室检测细胞侵袭和迁移。结果 SOX9 siRNA组细胞中SOX9 mRNA和蛋白水平均明显低于control组（PPP结论 下调SOX9可抑制OSCC细胞EMT和克隆形成,以及细胞侵袭和迁移。
英文摘要
ObjectiveTo investigate the effect of sex determining region Y-box 9 (SOX9) on epithelial mesenchymal transition (EMT) and cloning of oral squamous cell carcinoma (OSCC).MethodssiRNA control, SOX9 siRNA were trans-fected into BcaCD885 cells in OSCC. Simultaneously, cells that did not undergo transfection were used as the control. Quanti-tative real time polymerase chain reaction (qRT-PCR) and Western blot were used to select SOX9 siRNA1 with enhanced interference effect. A cell cloning assay was used to determine the cell’s clone formation ability. E-cadherin and Vimentin expressions were detected by immunofluorescence. The expressions of E-cadherin, matrix metalloprotease 2 (MMP-2), Vi-mentin and matrix metalloprotease 9 (MMP-9) were detected by Western blot. Cell invasion and migration were detected in the Transwell compartment.ResultsThe levels of SOX9 mRNA and protein in SOX9 siRNA cells were significantly lower than those of the control (PPPConclusionDown-regulation of SOX9 inhibited EMT, clonogenic formation, cell invasion and OSCC migration.
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