辛伐他汀-胶原蛋白复合海绵用于大鼠磨牙直接盖髓的实验研究
An experimental study of simvastatin-collagen composite sponge as a direct pulp capping material in rat molars
作者：张杨安,关为群,李群,刘玲玲
Author：Yang’an Zhang,Weiqun Guan,Qun Li,Lingling Liu
收稿日期：2018-09-27 年卷（期）页码：2019,37(2):155-155-161
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：辛伐他汀,胶原蛋白,直接盖髓术,修复性牙本质,
Key words：simvastatin,collagen sponge,direct pulp capping,reparative dentin,
基金项目：福建省自然科学基金卫生联合资金(2016J01550)
中文摘要
目的 评估辛伐他汀-胶原蛋白复合海绵作为直接盖髓材料的疗效及可行性。方法 120只SD大鼠随机分成3组,即辛伐他汀-胶原蛋白海绵组（SIM组）、胶原蛋白海绵组（CS组）及氢氧化钙组（CH组）,每组40只。在每只大鼠左上颌第一磨牙咬合面开髓,分别用3种材料直接盖髓,玻璃离子充填,并设对侧上颌第一磨牙为正常对照组。在术后第1、3、7、14、28天处死大鼠后拍摄X线片、苏木精-伊红（HE）及Masson’s Thichrome染色,观察牙髓组织生物学反应及牙本质桥形成情况。结果 影像学上,CH组术后第28天见穿髓孔处有高密度阻射影且封闭穿髓孔;术后第14天和第28天,SIM组在穿髓孔处见有高密度阻射影且穿髓孔封闭;而CS组仅在穿髓孔远中见高密度钙化团块影像,但未见钙化组织将穿髓孔封闭。HE及Masson’s Thichrome染色结果显示：不同时间点,3组中观察到不同的炎症反应,CS组炎症程度最重,SIM组与CS组的炎症反应程度在术后第14、28天存在统计学差异（PPPP结论 辛伐他汀-胶原蛋白复合海绵生物相容性好,促进修复性牙本质形成能力较强,作为盖髓材料具有良好的应用前景。
英文摘要
ObjectiveTo assess the feasibility and efficacy of simvastatin-collagen composite sponge as a novel, direct pulp capping material.MethodsA total of 120 Sprague-Dawley rats were randomly divided into three groups: the simvastatin-collagen composite sponge group (SIM group), the collagen sponge group (CS group), and the Ca(OH)2group (CH group). An endodontic entry cavity was prepared on the occlusion of the first molar on the left maxillary of each rat. The contralateral teeth were utilized as the normal control group. The rats were experimented after 1, 3, 7, 14, and 28 days. X-ray observations were conducted and the specimens underwent hematoxylin-eosin (HE) and Masson’s Thichrome staining. Dentin bridge formations and pulpal biology reactions were evaluated histopathologically.ResultsX-ray results: high-density images could be observed on the pulp exposure sites in the CH group on the 28th day. In the SIM group, high-density images could be observed after 14 and 28 days, whereas in the CS group, high-density images were not observable in the exposed area. HE and Masson’s Thichrome staining results: different degrees of inflammation under the cavity were detected in the three groups at different time points. The inflammatory reaction of the CS group was the most serious. The degree of the inflammatory reaction varied significantly between the SIM and the CS groups on the 14th and 28th days (PPPPConclusionThe simvastatin-collagen composite sponge exhibited satisfactory biocompatibility with the pulp tissue and promoted the formation of reparative dentin. The application of simvastatin-collagen composite sponge as a pulp-capping material has satisfactory potential.
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