环状RNA hsa_circ_0002203对口腔鳞状细胞癌细胞增殖、迁移、侵袭和凋亡能力的影响
Effect of circular RNA hsa_circ_0002203 on the proliferation, migration, invasion, and apoptosis of oral squamous cell carcinoma cells
作者：苏文,王宇帆,王锋,杨辉俊,杨宏宇
Author：Su Wen,Wang Yufan,Wang Feng,Yang Huijun,Yang Hongyu
收稿日期：2019-03-25 年卷（期）页码：2019,37(5):509-509-515
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：口腔鳞状细胞癌,环状RNA hsa_circ_0002203,增殖,迁移,侵袭,
Key words：oral squamous cell carcinoma,circular RNA hsa_circ_0002203,proliferation,migration,invasion,
基金项目：国家自然科学基金（81572654）(81572654);深圳市三名工程项目(北京大学口腔医院口腔颌面外科俞光岩教授团队)(SZSM 20151-2036)
中文摘要
目的 探讨环状RNA hsa_circ_0002203对口腔鳞状细胞癌（OSCC）细胞系恶性生物学行为的影响。方法 纳入OSCC患者40例,使用实时荧光聚合酶链反应检测环状RNA hsa_circ_0002203在OSCC组织、癌旁组织、OSCC细胞系和人口腔黏膜角质形成细胞（HOK）中的表达水平。慢病毒感染SCC15和CAL27细胞,实时荧光聚合酶链反应检测环状RNA hsa_circ_0002203的表达,细胞计数（CCK-8）实验检测细胞增殖能力,划痕实验和Transwell迁移及侵袭实验检测细胞迁移侵袭能力,流式细胞凋亡实验检测细胞凋亡水平,蛋白质印迹法检测细胞增殖凋亡侵袭相关蛋白的表达。通过裸鼠成瘤实验观察hsa_circ_0002203对SCC15细胞体外成瘤能力的影响。结果 环状RNA hsa_ circ_0002203在OSCC组织的表达低于癌旁组织（PP结论 环状RNA hsa_circ_ 0002203在口腔鳞状细胞癌中的低表达可以增强肿瘤细胞的增殖、迁移和侵袭能力,抑制肿瘤细胞凋亡。
英文摘要
ObjectiveTo investigate the effect of circular RNA hsa_circ_0002203 on the malignant biological behavior of oral squamous cell carcinoma (OSCC) cell lines.MethodsForty patients with oral squamous cell carcinoma were included. Real-time fluorescent polymerase chain reaction (PCR） was used to detect the expression level of circular RNA hsa_circ_ 0002203 in OSCC and corresponding adjacent tissues, OSCC cell lines, and human oral keratinocytes (HOK). SCC15 and CAL27 cells were transfected with lentivirus. The expression level of circular RNA hsa_circ_0002203 was detected by real-time fluorescent PCR. Cell proliferation was detected by cell counting assay (CCK-8). Cell migration and invasion ability was detected by scratch assay and Transwell migration and invasion assay. Apoptosis level was detected by flow cytometry. The expression of corresponding protein was detected by Western blot. Murine tumor formation experiments were performed to observe the effect of hsa_circ_0002203 on the tumorigenesis of SCC15 cellsin vivo.ResultsThe expression of circular RNA hsa_circ_0002203 in OSCC tissues was lower than that in adjacent tissues (PPConclusionThe low expression of circular RNA hsa_circ_0002203 in oral squamous cell carcinoma can enhance the proliferation, migration, and invasion of cancer cells and inhibit tumor cell apoptosis.
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