长链非编码RNA肌动蛋白纤维相关蛋白1-反义RNA1在口腔鳞状细胞癌中的表达及其相关功能
Expression and functions of long non-coding RNA actin filament-associated protein 1-antisense RNA1 in oral squamous cell carcinoma
作者：耿玉东,王树斌,卢泰青,滕薇
Author：Geng Yudong,Wang Shubin,Lu Taiqing,Teng Wei
收稿日期：2019-03-11 年卷（期）页码：2019,37(6):594-594-601
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：口腔鳞状细胞癌,长链非编码RNA,肌动蛋白纤维相关蛋白1-反义RNA1,预后,增殖,侵袭,
Key words：oral squamous cell carcinoma,long non-coding RNA,actin filament-associated protein 1-antisense RNA1,prognosis,proliferation,invasion,
基金项目：
中文摘要
目的 分析长链非编码RNA（lncRNA）肌动蛋白纤维相关蛋白1-反义RNA1（AFAP1-AS1）在口腔鳞状细胞癌（OSCC）中的表达及其对OSCC细胞生物行为学的影响,初步探讨其可能的作用机制。方法 采用实时荧光定量聚合酶链反应（qRT-PCR）检测OSCC患者（55例）癌组织、癌旁正常黏膜组织及人口腔鳞状细胞癌SCC25、人正常口腔角质细胞株（NOK）细胞中lncRNA AFAP1-AS1的表达,分析AFAP1-AS1与OSCC患者病理特征的相关性,通过Kaplan-Meier生存曲线分析AFAP1-AS1与患者预后的关系。AFAP1-AS1 siRNA转染SCC25细胞,细胞计数（CCK-8）及Transwell实验分别检测细胞增殖、迁移和侵袭的变化,蛋白质印迹（Western blot）检测侵袭相关蛋白、肌动蛋白纤维相关蛋白1（AFAP1）及Rho GTP酶家族成员蛋白的表达情况,免疫荧光染色检测细胞骨架肌动蛋白微丝结构的变化。结果 OSCC组织中AFAP1-AS1的表达高于癌旁正常黏膜组织,SCC25细胞中AFAP1-AS1的表达高于NOK细胞（PPP结论 lncRNA AFAP1-AS1在OSCC组织及SCC25细胞中高表达,与OSCC的发生进展及预后相关。下调AFAP1-AS1能够抑制OSCC的增殖、迁移和侵袭能力,其可能是通过调控肌动蛋白纤维丝的完整性实现的。
英文摘要
ObjectiveTo analyze the expression and clinical significance of long non-coding RNA (lncRNA) actin filament-associated protein 1-antisense RNA1 (AFAP1-AS1) in oral squamous cell carcinoma (OSCC) and its effect on the biobehavior of OSCC cells.MethodsQuantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of lncRNA AFAP1-AS1 in the tumor tissue and matching adjacent normal tissue of OSCC patients (n=55), SCC25 cells, and normal oral keratinocyte lines (NOK) cells. The correlation between AFAP1-AS1 expression and the clinicopathological characteristics of OSCC patients was analyzed. The relationship between AFAP1-AS1 and prognosis was also studied with the Kaplan-Meier method. AFAP1-AS1 siRNA was transfected into the SCC25 cells. Cell counting kit-8 (CCK-8) and Transwell were used to detect cell proliferation, invasion, and migration. The expression of the invasion-associated protein, AFAP1, and Rho GTPase family members, was detected by Western blot. In addition, the immunofluorescence of the cytoskeletal actin filament was observed.ResultsThe expression of AFAP1-AS1 was higher in the OSCC tissues than in the NOK cells, and the relative expression of AFAP1-AS1 was higher in the SCC25 cells than in the NOK cells (PPPConclusionThe expression of AFAP1-AS1 was high in the OSCC tissues and SCC25 cells and is associated with the development and prognosis of OSCC. The knockdown of AFAP1-AS1 might inhibit the proliferation and invasion of OSCC cells by regulating the integrity of the actin filament.
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