改良小鼠胚胎下颌下腺的获取及其体外器官培养模型的建立
Acquisition of submandibular gland in mouse embryo and establishment of an in vitro organ culture model
作者：刘志凯, 乔翔鹤, 苟黎明, 李春洁
Author：Liu Zhikai, Qiao Xianghe, Gou Liming, Li Chunjie
收稿日期：2019-07-04 年卷（期）页码：2020,38(2):228-228-232
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：小鼠,胚胎,下颌下腺,体外器官培养,分支形态发生,
Key words：mice,embryo,submandibular gland,in vitro organ culture,branching morphogenesis,
基金项目：国家自然科学基金(81870782);四川大学创新火花项目(2018SCUH0055)
中文摘要
分支形态的形成对于保证器官可以在有限的体积内获得最有效的功能形态具有重要意义。下颌下腺是研究器官分支形态发生过程的重要模型,在小鼠胚胎中获取下颌下腺组织进行体外器官培养是一种重要的研究方法。本文介绍了一种改良的小鼠胚胎下颌下腺获取方法,并将整个获取及建立体外器官培养流程作以简介,以对其在体内发育过程中的分支形态发生过程进行更好体外的模拟,从而能够更好地开展相关研究。
英文摘要
Branching morphology is important to ensure that the organ can obtain the efficient functional morphology in a limited volume. The submandibular gland is a crucial model for studying the morphological processes of organ branches. Harvesting the submandibular gland from mouse embryo is also an essential research technique. In this paper, a modified method for obtaining the submandibular glands of mouse embryo was introduced, and the whole process of obtaining and establishingin vitroorgan culture was briefly introduced to accurately simulate branch morphogenesis forvivodevelopment and related research.
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