口腔扁平苔藓组织中白细胞介素35受体的表达及意义
The expression and significance of interleukin-35 receptor in oral lichen planus
作者：张玲艳, 王荣, 柳汀, 蔡扬
Author：Zhang Lingyan, Wang Rong, Liu Ting, Cai Yang
收稿日期：2019-12-19 年卷（期）页码：2020,38(5):513-513-518
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：口腔扁平苔藓,白细胞介素35,白细胞介素35受体,糖蛋白130,白细胞介素12受体β2;,信号转导和转录激活因子1,信号转导和转录激活因子4,
Key words：oral lichen planus,interleukin-35,interleukin-35 receptor,glycoprotein 130,interleukin-12 receptor β2,singal transducer and activator of transcription 1,singal transducer and activator of transcription 4,
基金项目：国家自然科学基金(81460104)
中文摘要
目的 通过检测白细胞介素35受体（IL-35R）两亚基糖蛋白130（gp130）、白细胞介素12受体β2（IL-12Rβ2）及信号转导和转录激活因子（STAT）1、STAT4在口腔扁平苔藓（OLP）组织中的表达,探讨IL-35R在OLP病损形成和发展中的作用及意义。方法 收集41例OLP组织（OLP组）和15例正常口腔黏膜组织（对照组）为研究对象。采用实时荧光定量聚合酶链反应检测组织中gp130、IL-12Rβ2、STAT1、STAT4 mRNA的表达水平,免疫组织化学（IHC）检测组织中gp130、IL-12Rβ2蛋白的表达及分布,并分析gp130、IL-12Rβ2蛋白表达与OLP临床特征的关系。结果 1）OLP组gp130、IL-12Rβ2、STAT1、STAT4 mRNA的相对表达量均高于对照组（P<0.05）。2）OLP组gp130、IL-12Rβ2蛋白的阳性表达率均高于对照组（P<0.05）,且gp130、IL-12Rβ2蛋白在OLP组织中的表达呈正相关（r=0.984,P<0.001）。3）糜烂型OLP组织gp130、IL-12Rβ2蛋白的阳性表达率高于非糜烂型（P<0.05）。结论 IL-35R及STAT在OLP组织中表达上调,且IL-35R的表达与OLP的临床分型有关,提示IL-35R可能在OLP病损形成及发展中起重要作用。
英文摘要
ObjectiveTo investigate the expression of glycoprotein 130 (gp130) and interleukin 12 receptor β2 (IL-12Rβ2) in two subunits of interleukin-35 receptor (IL-35R), singal transducer and activator of transcription (STAT) 1 and STAT4 in oral lichen planus (OLP) tissues, and to explore the role and significance of IL-35R in the formation and development of OLP lesions.MethodsTotally 41 samples of OLP tissues (OLP group) and 15 samples of normal oral mucosa (control group) were collected. The expression levels of gp130, IL-12Rβ2, STAT1, STAT4 mRNA in the tissues were detected by real-time fluorescent quantitative polymerase chain reaction and the distribution and expression of protein gp130 and IL-12Rβ2 were detected by immunohistochemistry. The potential relationship between gp130 and IL-12Rβ2 and clinical features of OLP was analyzed.Results1) The expression levels of gp130, IL-12Rβ2, STAT1 and STAT4 mRNA in the OLP group were significantly higher than those in the control group (P<0 .05). 2) the positive expression rates of gp130 and il-12rβ2 protein in the olp group were higher than those in the control group (P<0 .05). the expression of gp130 and il-12rβ2 proteins in olp tissues were positively correlated (r=0.984,P<0 .001). 3) the expression rates of gp130 and il-12rβ2 protein in erosive olp tissues were significantly higher than those in non-erosive ones (P<0 .05).ConclusionThe expression of IL-35R and STAT is up-regulated in OLP tissues, and the expression of IL-35R is related to the clinical classification of OLP, suggesting that IL-35R might play an important role in the formation and development of damage OLP lesions.
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