ObjectiveTo study the antitumor effect of piceatannol (PIC) on malignant melanomain vitroandin vivo.
MethodsB16F10 cells were culturedin vitroand treated with gradient concentrations of PIC. Cell viability was detected with methyl thiazolyl tetrazolium (MTT) assay; matrix metalloproteinase (MMP)-2, MMP-9, vascular endothelial growth factor (VEGF), spleen tyrosine kinase (Syk), and p-Syk were detected with Western blot; migration ability was detected with wound healing assay; invasion ability was detected with Transwell assay. Syk expression was suppressed through RNA interference for the detection of the possible mechanism of PIC in melanoma. Anin vivostudy was established by creating B16F10-bearing mice with intraperitoneal injection of PIC.
ResultsThe cell viability of B16F10 decreased with increasing PIC concentration. The results of the Transwell assay showed that invasion ability decreased with increasing PIC concentration, and healing time was prolonged at increased PIC concentration in the wound healing assay. Western blot results showed that PIC mainly inhibited the phosphorylation of Syk and inhibited the expression of MMP-2, MMP-9, and VEGF. RNA interference pointed out that blocking the expression of Syk can reveal the same inhibition effect on B16F10 cells as PIC.In vivostudy revealed that different concentrations of PIC cangreatly inhibit melanoma progression.
ConclusionPIC might block the progression of malignant melanoma by inhibiting spleen tyrosine kinase.
