微小RNA663b对口腔鳞状细胞癌细胞迁移、侵袭和上皮间充质转化的影响
Effect of microRNA-663b on migration, invasion and epithelial‑mesenchymal transition of oral squamous cell carcinoma cells
作者：丛碧桥, 刘晓萍, 陈嘉雯, 李洪利, 范欣
Author：Cong Biqiao, Liu Xiaoping, Chen Jiawen, Li Hongli, Fan Xin
收稿日期：2022-03-23 年卷（期）页码：2022,40(4):386-386-393
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：miR-663b,SH3BP2,口腔鳞状细胞癌,迁移,侵袭,上皮间质转化,
Key words：miR-663b,SH3BP2,oral squamous cell carcinoma,migration,invasion,epithelial-mesenchymal transition,
基金项目：国家自然科学基金青年科学基金项目(81702932);潍坊医学院附属医院种子基金资助(2021wffyzzjj06)
中文摘要
目的 探讨微小RNA663b（miR-663b）对口腔鳞状细胞癌（OSCC）细胞迁移、侵袭和上皮间质转化（EMT）的影响。方法 通过基因表达数据库（GEO）使用R Studio进行OSCC组织与癌旁正常组织的差异表达分析。实时荧光定量聚合酶链反应（qRT-PCR）检测miR-663b在组织和细胞中的表达。检测miR-663b敲除质粒的HN30细胞转染效率，Transwell法检测迁移、侵袭能力。生物信息学方法预测miR-663b的靶向mRNA，双荧光素酶实验验证结合情况。Western blot检测EMT相关标志物的表达。结果 miR-663b在OSCC组织中表达上调，在HN30、CAL27、SCC-9细胞中表达较HOEC细胞高（P<0.05）。敲除miR-663b可以抑制HN30细胞的迁移和侵袭（P<0.05），抑制EMT的发生。生信预测SH3BP2与miR-663b靶向结合，SH3BP2低表达的患者预后较差（P<0.05）。双荧光素酶实验证明miR-663b可以与SHBP2靶向结合（P<0.05）。敲除miR-663b的OSCC细胞中SH3BP2的表达增加，EMT的发生受到抑制。结论 敲除miR-663b可靶向调节SH3BP2抑制OSCC细胞的迁移、侵袭和EMT。
英文摘要
ObjectiveTo explore the effect of microRNA-663b (miR-6636) on migration, invasion and epithelial-mesenchymal transition (EMT) of oral squamous cell carcinoma cells (OSCC).MethodsUse R Studio of gene expression omnibus (GEO) database to analyze expressions of miR-663b in the OSCC and adjacent normal tissues. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of miR-663b in tissues and cells. The transfection efficiency of HN30 cells with miR-663b knockout plasmid was detected. Transwell assay was used to detect the effect of the migration and invasion ability. Bioinformatics method was used to predict the targeted mRNA that may bind to miR-663b and double luciferase assay was used to verify the binding. Western blot assay was used to detect the expression of EMT-related markers.ResultsThe expression of miR-663b was up-regulated in OSCC tissues and higher in HN30, CAL27 and SCC-9 cells than in HOEC cells (P<0 .05). knockout of mir-663b could inhibit migration and invasion of hn30 cells (P<0 .05) and inhibit the occurrence of emt. bioinformatics prediction software predicts that sh3bp2 was the target gene of mir-663b, and patients with low sh3bp2 expression had a poor prognosis (P<0 .05). mir-663b could bind to shbp2 (P<0 .05). the expression of sh3bp2 was increased and the occurrence of emt was inhibited in hn30 cells with mir-663b knocked out.ConclusionKnockout of miR-663b can inhibit the migration, invasion and EMT of OSCC by targeting SH3BP2.
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