酸脱钙组织苏木素-伊红染色前处理改良方案
Improvement program on pretreatment of acid decalcified tissue in hematoxylin-eosin staining
作者：姚莉洪, 张美, 黄美畅, 万梓欣, 张伟龙, 杨肖, 杨名仲, 陈宇, 汤亚玲
Author：Yao Lihong, Zhang Mei, Huang Meichang, Wan Zixin, Zhang Weilong, Yang Xiao, Yang Mingzhong, Chen Yu, Tang Yaling
收稿日期：2019-09-22 年卷（期）页码：2020,38(3):297-297-300
期刊名称：华西口腔医学杂志
Journal Name：West China Journal of Stomatology
关键字：酸脱钙标本,苏木素-伊红染色,浓氨水,饱和碳酸锂溶液,
Key words：acid decalcification specimens,hematoxylin-eosin staining,concentrated ammonia water,saturated lithium carbonate solution,
基金项目：四川大学华西口腔医院临床研究项目(LCYJ2019-8)
中文摘要
目的 研究牙、骨等硬组织酸脱钙标本苏木素-伊红（HE）染色前处理方法,改善硬组织因酸脱钙后pH变化而引起的切片质量欠佳及HE染色组织结构不清的现象。方法 20例含硬组织的口腔病理标本硝酸脱钙后分别进行常规处理、浓氨水浸泡处理、饱和碳酸锂溶液浸泡处理,比较不同处理方法下切片质量及HE染色效果。结果 与常规处理相比,饱和碳酸锂溶液处理标本切片完整,苏木素着色强、胞核染色清晰,胞浆染色鲜艳。结论 酸脱钙标本在脱水包埋之前,经饱和碳酸锂溶液浸泡处理能中和脱钙组织酸性环境,切片质量及HE染色效果较好,适用于口腔病理酸脱钙组织样本的苏木素-伊红染色前处理。
英文摘要
ObjectiveTo explore the treatment conditions of acid decalcified specimens and improve the poor quality of sections and unclear structure of hematoxylin-eosin (HE) staining caused by the change in pH in tooth and hard tissue after acid decalcification.MethodsA total of 20 cases of oral pathological specimens that contain hard tissues were decalcified and treated with routine treatment, concentrated ammonia water immersion treatment, and saturated lithium carbonate solution immersion treatment. The quality and HE staining effects of hard tissue sections treated with different methods were compared.ResultsCompared with routine treatment, lithium carbonate saturated solution treatment showed complete sections. Hematoxylin is strongly stained, the nucleus is clear, and the cytoplasm is bright.ConclusionSoaking acid decalcified specimens in lithium carbonate saturated solution before embedding in dehydration can neutralize the acidic environment of the tissue. The quality of sections and HE staining effect are improved and are suitable for the pretreatment of acid decalcified tissue samples of oral pathology.
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